DETD-35 is an inhibitor of the MEK-ERK , Akt , and STAT3 signaling pathways, which promotes cancer cell apoptosis ( Apoptosis ) and reduces cancer cell resistance to Vemurafenib. The IC 50 values of DETD-35 against wild-type and mutant melanoma cell lines B16-F10, MeWo, SK-MEL-2, A2058c, and A375c are 2.7, 6.0, 3.9, 3.1, and 2.5 μM, respectively. DETD-35 holds promise for research in the field of anti-melanoma therapy.