DNA Gyrase-IN-11 (Compound 23Be) is an inhibitor for protein synthesis ( IC 50 is 0.74 μM) and DNA replication. DNA Gyrase-IN-11 inhibits DNA gyrase, that inhibits E. coli DNA supercoiling with IC 50 of 11.9 μM. DNA Gyrase-IN-11 exhibits antibacterial efficacy, that inhibits Streptococcus pneumoniae , Streptococcus pyogenes , Haemophilus influenzae and Staphylococcus aureus , with MIC s of 0.008-0.25 μg/mL.