计算溶液所需的质量、体积或浓度。
D-半乳糖β1 - 3-N -乙酰-己糖胺磷酸化酶 (BiGalHexNAcP),EC 2.4.1.211,来源于Bifidobacterium infantis。大肠杆菌重组表达,该酶在 PmPPA,GalK共同作用下,一锅双酶合成 T 抗原,galacto- N-biosse (Galβ1-3GalNAc)、乳糖-N-biosse (Galβ1-3GlcNAc)及其衍生物。
反应式
GlcNAcOR+Gal-1-P→Galβ1-3GlcNAcOR+PPi
使用方法
按照反应体系加入底物,可适当加入缓冲盐如Tris-HCl,调节 pH 为 7.5,加入适量 BiGalHexNAcP(根据反应需求);
混匀后,将样品置于 37℃孵育 2-4h。
注
体系放大时候,孵育时间或酶量需要根据实际情况调整,可低速震荡混匀。
注意事项
为了您的安全和健康,请穿实验服并戴一次性手套操作。本产品仅作科研用途。
D‑galactosyl‑β1‑3‑N‑acetylhexosamine phosphorylase (BiGalHexNAcP), EC 2.4.1.211, derived from Bifidobacterium infantis. Recombinantly expressed in E. coli. Together with PmPPA and GalK, this enzyme enables one‑pot two‑enzyme synthesis of T‑antigen, galacto‑N‑biose (Galβ1‑3GalNAc), lacto‑N‑biose (Galβ1‑3GlcNAc) and their derivatives.
Reaction Equation
GlcNAcOR + Gal‑1‑P → Galβ1‑3GlcNAcOR + PPi
Usage
Add substrates to the reaction system. Appropriately add buffer salt such as Tris‑HCl to adjust pH to 7.5, then add an appropriate amount of BiGalHexNAcP according to reaction requirements. After mixing, incubate the sample at 37 °C for 2‑4 h.
Note
For scaled‑up reactions, incubation time or enzyme dosage should be adjusted according to practical conditions; low‑speed shaking mixing is recommended.
Precautions
For your safety and health, wear a lab coat and disposable gloves during operation.
This product is for research use only.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | D1439090 | |
| 分析证书 | D1439090 |
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