DDO-02267 is a selective and lysine-targeting covalent inhibitor of ALKBH5 , with an IC 50 value of 0.49 μM. DDO-02267 increases N 6 -methyladenosine (m 6 A) levels and targets the ALKBH5-AXL signaling axis. DDO-02267 can serve as a probe for investigating the biological function of mRNA demethylase.