The carbohydrate binding site recognizes (β-1,4) linked N-acetylglucosamine oligomers, preferring chitobiose or chitotriose over a single N-acetylglucosamine residue. This lectin binds well in the acidic pH range but its affinity decreases above pH 8.0. DSL also binds well to N-acetyllactosamine and oligomers containing repeating N-acetyllactosamine sequences. A branched pentasaccharide including two N-acetyllactosamine disaccharides linked to mannose (β-1,6) and (β-1,2) was reported to be the most potent inhibitor of agglutination.
DSL contains two chains of 40 kDa and 46 kDa joined by disulfide bonds. This lectin is free of a reported 32 kDa contaminant protein.
Inhibiting/Eluting Sugar: Chitin Hydrolysate
Specifications
Molecular Weight
86 kDa
Extinction Coefficient
0.6
Inhibiting or Eluting Sugar
Chitin Hydrolysate or Desialylated Fetuin
Storage Instructions
2-8 °C; for long term storage, aliquots may be stored frozen or preserved with 0.08% sodium azide in the recommended buffer and stored at 2-8 °C.
Sugar Specificity
Branching structures with polyLacNAc
Usage Summary
Though many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl₂ is recommended. For preserving solutions stored at 4 °C, 0.08% sodium azide can be used. Aggregation may occur with time if stored at concentrations greater than 2 mg/ml.
Applications
Glycobiology, Mitogenic Stimulation
Conjugate
Unconjugated
规格
产品名称
曼陀罗凝集素 (DSL)
英文别名
Lectin from Datura stramonium
规格或纯度
BioReagent, 天然
种属
Datura stramonium
偶联
Unconjugated
来源
天然
预测分子量
86 kDa
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
复溶
Reconstitute in 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl₂. For preserving solutions stored at 4 °C, 0.08% sodium azide can be used. Aggregation may occur with time if stored at concentrations greater than 2 mg/ml.