储存缓冲液：Lyophilized from bulk protein in a 0.22 μm filtered solution of 0.01 M PB, 0.15 M NaCl, 3% trehalose, pH=7.4.
复溶：Reconstitute in ultrapure water.
阿拉丁生产的DBCO修饰的链霉亲和素 (DBCO-SA)是一种即用型的点击化学偶联试剂，采用高纯度链霉亲和素与DBCO-PEG5-NHS ester共价修饰而成。该产品可与末端叠氮修饰的抗体、寡核苷酸、聚合物或其它分子在温和条件下实现快速、高效的SPAAC 反应。
链霉亲和素具有生物学特性，每个分子由 4 个亚基构成，能够与 4 个生物素分子紧密结合，这种强相互作用的亲和常数高达 10¹⁵mol/L ，在生物检测、分离以及靶向传递等众多领域应用。而DBCO（二苯基环辛炔）是一种环炔烃，能够与叠氮化物在温和条件下发生高效的、无铜催化的点击化学反应，这种反应具有高选择性、快速动力学和良好的生物相容性，适用于活细胞和体内环境。聚乙二醇（PEG）链的引入则增强了试剂的溶解性、稳定性和生物相容性，减少非特异性结合，延长循环半衰期。DBCO-SA 融合了链霉亲和素与DBCO基团的优势，在生物分子的标记、偶联操作中，可先利用链霉亲和素与生物素化的分子特异性结合，再借助DBCO基团与叠氮修饰分子的点击反应，实现多种生物分子的准确连接，为生物研究提供了便捷且工具，并且整个反应过程条件相对温和，对生物分子的原有活性影响较小。
该DBCO-SA产品通过尺寸排阻色谱法进行纯化，去除了未偶联的分子，确保其在体外及体内应用中均具有良好的适用性。
每个链霉亲和素蛋白上大约含有4-9个DBCO基团。
使用建议
储存条件：冻干粉在2-8℃干燥、避光长期保存；复溶后溶液建议4℃保存不超过1周，或-20℃分装冻存。
LumiDye™ Dibenzocyclooctyne-streptavidin (DBCO-SA) is a ready-to-use click chemistry conjugation reagent, prepared by covalently modifying high-purity streptavidin with DBCO-PEG5-NHS ester. This product enables rapid and efficient copper-free strain-promoted alkyne-azide cycloaddition (SPAAC) with terminal azide-modified antibodies, oligonucleotides, polymers, or other molecules under mild conditions.
Streptavidin possesses distinct biological properties: each molecule consists of four subunits capable of binding four biotin molecules with extremely high affinity, exhibiting a binding constant as high as 10¹⁵ M⁻¹, making it widely applicable in areas such as biological detection, separation, and targeted delivery, and many other fields. DBCO (dibenzocyclooctyne) is a cyclooctyne that undergoes efficient, copper-free click chemistry with azides under mild conditions. This reaction features high selectivity, fast kinetics, and excellent biocompatibility, making it suitable for live-cell and in vivo applications. The introduction of a polyethylene glycol (PEG) spacer enhances the reagent’s solubility, stability, and biocompatibility, reduces non-specific binding, and prolongs circulation half-life. DBCO-SA combines the advantages of streptavidin and DBCO groups. In biomolecular labeling and conjugation workflows, DBCO-SA first utilizes the specific binding between streptavidin and biotinylated molecules, followed by the click reaction between the DBCO group and azide-modified molecules, enabling accurate linkage of various biomolecules. This provides a convenient tool for biological research, and the entire process occurs under mild conditions with minimal impact on the native activity of the biomolecules.
The DBCO-SA was purified by size chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.
There are approximately 4-9 DBCO groups on a streptavidin protein.
Usage Suggestion
Storage conditions: The lyophilized powder should be stored dry and protected from light at 2–8°C for long-term preservation. After reconstitution, the solution is recommended to be stored at 4°C for no more than 1 week, or aliquoted and frozen at –20°C.
ZJ26F0535838
危险品化学品经营许可证（带存储）