BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
二胺氧化酶（DAO, EC1.4.3.6）广泛存在于动物（肠粘膜、肺、肝脏、肾脏等）、植物和微生物中。DAO 催化多胺氧化为醛，其活性与核酸和蛋白合成密切相关，能够反映肠道机械屏障的完整性和受损伤程度。该试剂盒可检测生物体内 DAO 活性，其原理是 DAO 催化尸胺产生醛和过氧化氢，外源添加过量的辣根过氧化物酶，催化过氧化氢氧化邻联茴香胺生成有色物质，在 460 nm 处有特征吸收峰，通过测定该波长吸光度增加速率，计算DAO活性。
Diamine Oxidase (DAO, EC 1.4.3.6) is widely present in animals (intestinal mucosa, lungs, liver, kidneys, etc.), plants, and microorganisms. DAO catalyzes the oxidation of polyamines to aldehydes. Its activity is closely related to nucleic acid and protein synthesis and can reflect the integrity and extent of damage to the intestinal mechanical barrier. This assay kit is designed to detect DAO activity in biological systems. The detection principle is as follows: DAO catalyzes the oxidation of cadaverine, producing an aldehyde and hydrogen peroxide. With the exogenous addition of an excess amount of horseradish peroxidase (HRP), HRP then catalyzes the reaction where hydrogen peroxide oxidizes *o*-dianisidine to generate a colored product. This product exhibits a characteristic absorption peak at 460 nm. By measuring the rate of increase in absorbance at this wavelength, the DAO activity can be calculated.
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