计算溶液所需的质量、体积或浓度。
BioReagent,无菌过滤,用于细胞培养 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
来源于我国呼伦贝尔和锡林郭勒天然牧场6~8月龄健康剖腹产胎牛的血液,通过无菌采集后进行透析处理。透析过程通常使用0.15M NaCl溶液,去除分子量小于10000 Da的小分子物质,如葡萄糖、氨基酸、盐类和非结合蛋白分子。此外,产品还经过三次0.1微米过滤,确保无菌性。
适用范围:
1) 提供大分子蛋白和低分子营养素。
2) 增强缓冲能力,结合或中和有毒成分。
3) 减少细胞氧化应激和凋亡。
4) 提供生长因子,促进细胞适应培养环境。
5) 适用于蛋白质组学、同位素标记、细胞信号传导和报告基因分析等研究领域。
6) 适用于药物筛选和克隆筛选。
7) 适用于糖代谢、放射性标记研究、凋亡研究、锌摄取动力学研究等需要小分子浓度控制的实验中。
产品特点:
1) 消除外源性小分子干扰,适用于对代谢研究敏感的细胞培养。
2) 保留大分子蛋白(如生长因子、粘附因子),维持细胞增殖能力。
3) 血清中葡萄糖含量<50 mg/L,内毒素水平≤9EU/mL,蛋白含量:30-45 g/L、pH值:7.0-8.0、渗透压250-330 mOsm/kg。
使用方法:
1) 本品经过0.1微米过滤除菌,无需再次过滤除菌。
2) 为避免沉淀增多,血清在融化过程中,须间歇的摇晃均匀。
3) 若一次无法用完1整瓶,建议在无菌环境下分装,-20℃冷冻保存,避免反复冻融。
注意事项:
如有以下情况,请勿使用
1) 包装有破损。
2) 溶液浑浊,有污染的疑虑。
3) 使用时已超出规定有效期。
4) 仅用于研究和进一步生产,不得直接用于人或动物。
Derived from the blood of healthy 6–8 month‑old caesarean‑derived fetal bovines from natural pastures in Hulunbuir and Xilingol League, China. The serum is collected aseptically and subjected to dialysis treatment. Dialysis is typically performed using 0.15 M NaCl solution to remove small molecules below 10 000 Da, such as glucose, amino acids, salts, and non‑bound protein molecules. In addition, the product is filtered three times through 0.1 μm membranes to ensure sterility.
Scope of Application:
1. Supplies macromolecular proteins and low‑molecular‑weight nutrients.
2. Enhances buffering capacity, binds or neutralizes toxic components.
3. Reduces cellular oxidative stress and apoptosis.
4. Provides growth factors and promotes cell adaptation to the culture environment.
5. Suitable for research fields including proteomics, isotope labeling, cell signaling, and reporter gene assays.
6. Suitable for drug screening and clone screening.
7. Suitable for experiments requiring controlled small‑molecule concentrations, such as glucose metabolism studies, radiolabeling, apoptosis research, and zinc uptake kinetics.
Product Features:
1. Eliminates interference from exogenous small molecules; suitable for cell culture sensitive to metabolic studies.
2. Retains macromolecular proteins (e.g., growth factors, adhesion factors) to maintain cell proliferation.
3. Glucose < 50 mg/L; endotoxin ≤ 9 EU/mL; protein 30–45 g/L; pH 7.0–8.0; osmolality 250–330 mOsm/kg.
Instructions for use:
1. This product has been 0.1 μm filter‑sterilized; no further filtration is required.
2. To prevent excessive precipitation, the serum must be gently shaken intermittently during thawing.
3. If one bottle cannot be used up at once, aliquot under aseptic conditions and store frozen at −20 °C; avoid repeated freeze‑thaw cycles.
Precautions:
Do NOT use if:
1. The package is damaged.
2. The solution is turbid or suspected of contamination.
3. The product has exceeded the stated expiry date.
4. For research and further manufacturing use only; not for direct use in humans or animals.
| pH | 7.0-8.0 |
|---|
通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | D1521106 | |
| 分析证书 | D1521106 |
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