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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
脱氢酶(dehydrogenase, DHA) 是一类催化物质氧化还原反应的酶,催化底物通过细胞色素系统被氧化,释放的能量供机体使用,是生物体取得能量的一种方式。 测定原理:在细胞呼吸过程中,氢受体2, 3, 5 - 氯化三苯基四氮唑(2,3,5-Triphenyl Tetrazolium Chloride, TTC)在脱氢酶作用下接受氢以后,被还原为三苯基甲鐟(Triphenyl Formazone, TF),TF呈现红色,于485nm测定其吸光值,即得脱氢酶活性。
Dehydrogenase (DHA) is a class of enzymes that catalyze oxidation-reduction reactions. It oxidizes substrates through the cytochrome system, releasing energy for use by the organism, representing a key mechanism by which organisms obtain energy. Determination principle: During cellular respiration, the hydrogen acceptor 2,3,5‑triphenyltetrazolium chloride (TTC) accepts hydrogen under the action of dehydrogenase and is reduced to triphenylformazan (TF), which appears red. The dehydrogenase activity is obtained by measuring its absorbance at 485 nm.