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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
AsA作为植物细胞一个重要生理指标,其AsA 的含量、氧化还原状态(AsA/DHA 比率)及其合成与代谢相关酶类活性的变化涉及植物对一系列环境胁迫的响应。DHA是AsA的可逆的氧化型,在生物体内,与抗坏血酸共同组成氧化还原系统,具有电子受体的作用。 测定原理:DTT还原DHA生成AsA,通过测定体系中AsA的生成速率,即可计算出DHA含量。
AsA is an important physiological indicator in plant cells. Changes in its content, redox state (AsA/DHA ratio), and the activities of enzymes involved in its synthesis and metabolism are associated with plant responses to various environmental stresses. DHA is the reversible oxidized form of AsA; in vivo, it constitutes a redox system together with ascorbic acid and functions as an electron acceptor. Assay principle: DTT reduces DHA to AsA. The DHA content can be calculated by measuring the rate of AsA generation in the system.