BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
维生素C(Vitamin C)又称L-抗坏血酸(AsA)，是高等灵长类动物与其他少数生物的必需营养素，在生物体内维生素C是一种抗氧化剂，为酸性己糖衍生物，是稀醇式己糖酸内酯，保护身体免于自由基的威胁，同时也是一种辅酶，其广泛的食物来源为各类新鲜蔬果。Vc有L-型和D-型两种异构体，只有L-型的才具有生理功能，还原型和氧化型都有生理活性。 该试剂盒检测原理是利用还原剂将脱氢抗坏血酸还原成还原型抗坏血酸，在酸性条件下还原型抗坏血酸把三价铁离子还原成亚铁离子，后者与菲咯啉形成稳定的红色螯合物，以酶标仪534nm处检测吸光度，在一定浓度范围(样品浓度控制在1~40μg/ml)吸光度与抗坏血酸含量呈线性关系，获得抗坏血酸含量。该试剂盒主要用于植物组织中的维生素C(抗坏血酸)的检测，计算出总抗坏血酸含量，从中减去样品中原有的还原型抗坏血酸含量，即得脱氢抗坏血酸含量。优点是：1、反应稳定，不易褪色；2、操作简便；3、还原糖及其他常见的还原物质对实验没有干扰，因此专一性好；4、灵敏度高。本产品仅用于科研领域，不宜用于临床诊断或其他用途。
Vitamin C (Vitamin C), also known as L-Ascorbic Acid (AsA), is an essential nutrient for higher primates and a few other organisms. In living organisms, Vitamin C acts as an antioxidant; it is an acidic hexose derivative and a lactone of an enolic hexuronic acid, protecting the body from damage by free radicals. It also functions as a coenzyme. Its widespread dietary sources are various fresh fruits and vegetables. Vitamin C has two isomers, the L-form and the D-form, but only the L-form possesses physiological function. Both the reduced and oxidized forms exhibit physiological activity. The detection principle of this kit is as follows: A reducing agent is used to reduce dehydroascorbic acid to reduced ascorbic acid. Under acidic conditions, the reduced ascorbic acid reduces ferric ions to ferrous ions, which then form a stable red chelate complex with phenanthroline. The absorbance is measured at 534 nm using a microplate reader. Within a certain concentration range (sample concentration controlled between 1-40 μg/ml), the absorbance shows a linear relationship with the ascorbic acid content, allowing for the determination of ascorbic acid concentration. This kit is primarily used for detecting Vitamin C (ascorbic acid) in plant tissues to calculate the total ascorbic acid content. By subtracting the original reduced ascorbic acid content present in the sample, the dehydroascorbic acid content can be obtained. The advantages of this method are: 1. Stable reaction with minimal fading; 2. Simple operation; 3. No interference from reducing sugars or other common reducing substances, ensuring good specificity; 4. High sensitivity. This product is for research use only and is not intended for use in diagnostic procedures.
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