计算溶液所需的质量、体积或浓度。
BioReagent,适用于植物细胞培养 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
DPD培养基是专门适配木本植物培养的专用基础干粉培养基,无机盐与有机营养配比均衡,渗透压温和, 重复性好、适配性强,针对性适配树木类植物生理特性,是木本植物原生质体培养、生根培养等的经典培养基配方。
产品成分:
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使用方法
1、配制溶解:精确称取本品77.6 g,加入1 L蒸馏水或去离子水中,充分搅拌至粉末完全溶解,配制成均匀的培养基溶液。
2、pH调节(可选):如需调节pH值,可在灭菌前使用稀NaOH或者稀HCl溶液进行微调。植物组织培养常用pH范围为5.5~5.8,具体以实际实验方案为准。调节时逐滴添加、边加边搅拌并实时检测pH,避免过度调节。
3、灭菌处理:将配制完成的培养基分装至合适培养容器,于116 ℃高压蒸汽灭菌30 min。
4、冷却使用:灭菌结束后置于洁净无菌环境中自然冷却,全程严格遵守无菌操作规范,即可用于植物组织培养实验。
注意事项
1、本品为植物组织培养全无机盐培养基,建议采用室温水溶解后再进行高压灭菌,不建议加热溶解;灭菌后溶液若出现少量沉淀为正常现象,不影响实验使用。
2、产品需置于室温、避光环境密封保存,有效期3年。
3、本产品仅供科研实验使用,严禁用于医药、临床诊断与治疗、食品、化妆品等相关领域,请勿存放于普通住宅区。
4、因用户非正常操作、违规使用本产品所产生的一切意外与损失,本公司不承担相关责任。
NLN Medium (Calcium Nitrate Free) excludes calcium nitrate from its formulation while maintaining complete proportions of all other nutritional components. Calcium nitrate can be supplemented as required during preparation to effectively prevent precipitation. Featuring flexible formulation adjustment, it is commonly applied for in vitro tissue culture of oil crops and Brassicaceae plants.
Product Composition:
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Instructions for Use:
1. Dissolution: Accurately weigh 77.6 g of this product, add it into 1 L of distilled or deionized water, and stir thoroughly until completely dissolved to prepare a homogeneous medium solution.
2. pH Adjustment (Optional): If pH adjustment is required, finely tune the pH with dilute NaOH or dilute HCl solution before sterilization. The commonly applicable pH range for plant tissue culture is 5.5~5.8, subject to specific experimental protocols. Add the solution dropwise while stirring and monitor the pH in real time to avoid over-adjustment.
3. Sterilization: Dispense the prepared medium into suitable culture vessels and perform high-pressure steam sterilization at 116 ℃ for 30 minutes.
4. Cooling and Application: After sterilization, cool the medium naturally to room temperature in a clean and sterile environment. Use it for plant tissue culture experiments with strict aseptic operation.
Precautions:
1. This product is a full inorganic medium for plant tissue culture. It is recommended to dissolve the powder at room temperature before high-pressure sterilization; heating dissolution is not advised. A small amount of precipitate after sterilization is normal and will not affect the experimental effect.
2. Store the product in a sealed, room temperature and dark environment. The shelf life is 3 years.
3. This product is for research use only. Do not use it for medical treatment, clinical diagnosis, food, cosmetics or other commercial purposes. Do not store it in residential areas.
4. The company shall not be liable for any accidents or losses caused by abnormal or improper use of this product.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | D1524304 |
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 BioReagent 级别指南 → 查看 Suitable for plant cell culture 级别指南 →