DMEM (Dulbecco’s Modified Eagle Medium) is developed on the basis of MEM medium. Compared with MEM, it contains 2‑fold higher amino acids and 4‑fold higher vitamins, and is additionally supplemented with non‑essential amino acids, trace iron ions, and sodium pyruvate. DMEM was originally formulated with a glucose concentration of 1000 mg/L (low‑glucose type); later, a high‑glucose version containing 4500 mg/L glucose was also developed, and the medium is now widely used for various cell cultures.
High‑glucose DMEM is commonly applied for fast‑growing, low‑adherent cells, hybridoma and myeloma cells, clonal cells, transformed cells for DNA transfection, various primary virus host cells, single‑cell cultures, and vaccine production – for example, in CHO cells for EPO expression and hepatitis B vaccine manufacturing.
This product contains multiple components required for cell culture, including amino acids, vitamins, inorganic salts, etc.; however, it contains no proteins, lipids, or any growth factors. Therefore, this product must be used with serum or serum‑free supplements.
Precautions
1. This product has been sterilized by filtration. Aseptic techniques must be observed during handling to prevent contamination.
2. To maintain optimal performance, avoid freeze‑thaw cycles.
3. This product is intended for research or further study purposes only; it is not for diagnostic or therapeutic use.
规格
规格或纯度
BioReagent
英文名称
DMEM High Glucose Medium (without Sodium Pyruvate, with Penicillin-Streptomycin)
危险品化学品经营许可证（带存储）