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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Dulbecco's Modified Eagle Medium (DMEM) 是一种广泛使用的基础培养基,可用于支持很多种类的哺乳动物细胞生长。已经在 DMEM 培养基中培养成功的细胞包括原代成纤维细胞、神经元、胶质细胞、HUVEC 以及平滑肌细胞等原代细胞,还包括 HeLa、293、Cos-7和 PC-12等细胞系。我们提供了多种不同的DMEM改良培养基,适合广泛的细胞培养应用。 DMEM不含蛋白、脂类或生长因子。因此,DMEM 需要补充营养成分,通常需要添加 10% 胎牛血清,DMEM使用碳酸氢钠缓冲体系 (3.7 g/L),因此需要 5-10% CO2 的环境来维持生理 pH 值。 注意事项 1、本产品经过滤除菌,使用时应注意无菌操作,避免污染; 2、为保持本产品的最佳使用效果,请勿进行冻融处理; 3、本产品仅用于科研或进一步研究使用,不用于诊断和治疗。
Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium capable of supporting the growth of many types of mammalian cells. Cells successfully cultured in DMEM include primary cells such as primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines including HeLa, 293, Cos‑7, and PC‑12. We offer a variety of DMEM modifications suitable for a broad range of cell culture applications. DMEM contains no proteins, lipids, or growth factors. Therefore, it requires supplementation with nutrients, typically with 10% fetal bovine serum (FBS). DMEM utilizes a sodium bicarbonate buffering system (3.7 g/L) and thus requires a 5–10% CO₂ atmosphere to maintain physiological pH. Precautions ① This product has been sterilized by filtration. Aseptic techniques must be observed during handling to prevent contamination. ② To maintain optimal performance, avoid freeze‑thaw cycles. ③ This product is intended for research or further study purposes only; it is not for diagnostic or therapeutic use.