计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
本试剂盒提供一种快速、灵敏的D-乳酸检测方法:D-乳酸在D-乳酸脱氢酶(D-LDH)催化下生成丙酮酸,同时将NAD⁺还原为NADH;为驱动反应正向进行,体系中另添加辅助酶以进一步分解丙酮酸,使反应持续向右进行。生成的NADH与特异性显色剂反应,产生在450 nm处具有最大吸收峰的粉红色有色物质,通过测定该波长下吸光值的增加量,即可计算样品中D-乳酸的含量。
This kit provides a rapid and sensitive method for the determination of D-lactate. D-Lactate is catalyzed by D-lactate dehydrogenase (D-LDH) to generate pyruvate, while simultaneously reducing NAD⁺ to NADH. To drive the reaction forward, a coupling enzyme is included in the system to further convert pyruvate, thereby pushing the reaction continuously toward the product side. The generated NADH reacts with a specific chromogenic reagent, yielding a pink-colored product with a maximum absorbance peak at 450 nm. By measuring the increase in absorbance at this wavelength, the D-lactate content in the sample can be calculated.