BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
二胺氧化酶（DAO，EC 1.4.3.6）广泛存在于动物、植物及微生物中，催化二胺氧化生成醛，其活性与核酸和蛋白质合成密切相关，并与植物逆境生理有一定关联。本试剂盒采用比色法测定DAO活性：DAO催化二胺底物氧化生成相应的醛和过氧化氢（H₂O₂）；在过氧化物酶（POD）催化下，H₂O₂与4-氨基安替比林（4-AAP）及酚类显色剂发生氧化偶联反应，生成红色醌亚胺化合物，该产物在510 nm处具有特征吸收峰。通过测定该波长下吸光值的增幅，结合标准曲线即可精准计算样品中DAO的酶活力大小。
Diamine oxidase (DAO, EC 1.4.3.6) is widely distributed in animals, plants, and microorganisms. It catalyzes the oxidation of diamines to generate aldehydes. Its activity is closely associated with nucleic acid and protein synthesis, and is also related to the physiological responses of plants to environmental stress. This kit employs a colorimetric method for the determination of DAO activity: DAO catalyzes the oxidation of diamine substrates to produce the corresponding aldehydes and hydrogen peroxide (H₂O₂). In the presence of peroxidase (POD), H₂O₂ undergoes an oxidative coupling reaction with 4-aminoantipyrine (4-AAP) and a phenolic chromogenic reagent to form a red quinone imine compound, which exhibits a characteristic absorbance peak at 510 nm. By measuring the increase in absorbance at this wavelength and referencing a standard curve, the DAO enzyme activity in the sample can be precisely calculated.
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