BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
二胺氧化酶（DAO，EC 1.4.3.6）广泛存在于动物、植物及微生物中，催化二胺氧化生成醛，其活性与核酸和蛋白质合成密切相关。本试剂盒采用NADH偶联酶法测定DAO活性：DAO催化二胺底物氧化生成相应的醛和氨（NH₃）；生成的氨在谷氨酸脱氢酶（GDH）催化下，与α-酮戊二酸和还原型辅酶Ⅰ（NADH）反应生成谷氨酸，同时将NADH氧化为NAD⁺。通过监测340 nm处NADH特征吸收峰的下降速率，在底物饱和条件下，单位时间内OD₃₄₀的初始线性降幅（ΔOD₃₄₀/min）与DAO酶活性呈正比，结合标准曲线即可精准计算样品中DAO的酶活力大小。
Diamine oxidase (DAO, EC 1.4.3.6) is widely distributed in animals, plants, and microorganisms. It catalyzes the oxidation of diamines to generate aldehydes, and its activity is closely associated with nucleic acid and protein synthesis. This kit employs an NADH‑coupled enzymatic method for the determination of DAO activity: DAO catalyzes the oxidation of diamine substrates to produce the corresponding aldehydes and ammonia (NH₃). The generated ammonia, in the presence of glutamate dehydrogenase (GDH), reacts with α‑ketoglutarate and reduced nicotinamide adenine dinucleotide (NADH) to form glutamate, while simultaneously oxidizing NADH to NAD⁺. By monitoring the rate of decrease in the characteristic absorbance peak of NADH at 340 nm, under substrate‑saturated conditions, the initial linear decrease in OD₃₄₀ per unit time (ΔOD₃₄₀/min) is proportional to DAO enzyme activity. By referencing a standard curve, the DAO enzyme activity in the sample can be precisely calculated.
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