BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
本试剂盒为二苯胺微量法DNA定量检测试剂盒，配套包含DNA标准溶液、二苯胺显色液及乙醛增效液，适用于科研样本中总DNA的生化法定量检测。 检测原理：核酸分子经加热释放出2'-脱氧核糖，在酸性条件下脱水生成ω-羟基-γ-酮基戊醛；该产物与二苯胺试剂发生特异性显色反应，生成稳定的蓝色化合物。显色产物在595 nm处具有最大吸收峰，在DNA浓度20～400 μg/mL范围内，吸光度与DNA浓度呈良好线性关系（R²≥0.99）。反应体系中添加微量乙醛，可显著提升显色灵敏度，降低检测误差。 本试剂盒采用微孔板检测体系，兼容酶标仪高通量检测，操作便捷、重复性好，可通过标准曲线精准计算样本DNA含量，广泛适用于动植物组织、细胞及微生物样本的DNA定量分析。仅限科研使用，不可用于临床诊断、食品检测及其他非科研用途。
This kit is a diphenylamine microplate colorimetric assay for DNA quantification, including DNA standard solution, diphenylamine chromogenic reagent, and acetaldehyde enhancer solution. It is designed for the biochemical quantification of total DNA in research samples. Principle: Nucleic acid molecules release 2'-deoxyribose upon heating, which undergoes dehydration under acidic conditions to form ω-hydroxy-γ-ketopentanal. This product specifically reacts with diphenylamine reagent to generate a stable blue-colored compound. The chromogenic product exhibits a maximum absorbance at 595 nm. Within the DNA concentration range of 20–400 μg/mL, the absorbance shows a good linear correlation with DNA concentration (R² ≥ 0.99). The addition of trace amounts of acetaldehyde significantly enhances chromogenic sensitivity and reduces detection errors. This kit utilizes a microplate assay format, compatible with high-throughput microplate reader detection. It offers convenient operation and good reproducibility, and enables accurate calculation of sample DNA content via a standard curve. It is widely applicable for DNA quantification in animal and plant tissues, cells, and microbial samples. For research use only. Not for clinical diagnosis, food testing, or other non-research applications.
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