BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
本试剂盒基于经典二苯胺比色法原理，用于样本中脱氧核糖核酸（DNA）的定量检测。在强酸性加热条件下，DNA中的α-脱氧核糖发生脱水异构，生成ω-羟基-γ-酮基戊醛，该产物可与二苯胺试剂发生特异性显色反应，生成稳定的蓝色复合物。 该显色产物在595 nm波长处具有最大吸收峰。在DNA浓度20～400 μg/mL范围内，溶液吸光度与DNA浓度呈现良好的线性正比关系，可精准用于DNA含量定量测定。反应体系中添加微量乙醛，能够显著提升显色灵敏度，降低检测下限，提高检测准确度与重复性。 本试剂盒操作简便、成本低、稳定性好，适用于动植物组织、微生物、细胞样本的总DNA含量测定，广泛应用于生物化学、分子生物学及普通科研教学实验。仅限科研使用，不可用于临床诊断、食品检测及其他非科研用途。
This kit is based on the classical diphenylamine colorimetric method for the quantitative determination of deoxyribonucleic acid (DNA) in various samples. Under strong acidic and heating conditions, the α-deoxyribose in DNA undergoes dehydration and isomerization to form ω-hydroxy-γ-ketopentanal, which specifically reacts with diphenylamine reagent to generate a stable blue-colored complex. The chromogenic product exhibits a maximum absorbance at 595 nm. Within the DNA concentration range of 20–400 μg/mL, the absorbance shows a good linear correlation with DNA concentration, enabling accurate quantification of DNA content. The addition of trace amounts of acetaldehyde to the reaction system significantly enhances detection sensitivity, lowers the detection limit, and improves both accuracy and reproducibility. This kit offers advantages including simple operation, low cost, and good stability. It is suitable for the determination of total DNA content in animal and plant tissues, microorganisms, and cultured cells, and is widely used in biochemistry, molecular biology, and general research and teaching laboratories. For research use only. Not for clinical diagnosis, food testing, or other non-research applications.
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