BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
阿拉丁葡聚糖羧基磁珠 (50nm)，具有超顺磁性、悬浮稳定性好、单分散性好、抗非特异性吸附强等特点。磁珠表面富含羧基，可与蛋白、抗体、肽、寡核苷酸等共价偶联，适用于免疫检测、亲和纯化、细胞分选等领域。磁珠在不同缓冲溶液中具有优良的悬浮稳定性，能有效确保反应的均一性和检测一致性。
产品信息
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参考偶联方案，以 SA 为例
A．试剂准备
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B．磁珠准备及清洗
1．充分混匀磁珠，取10 mg 磁珠至离心管中，磁分离弃上清，加入1 mL 偶联缓冲液重悬，水浴超声20 s或涡旋混匀，采用偶联缓冲液重复洗涤1次后定容至10 mg/mL（重悬至1 mL）；
C．SA 包被及磁珠封闭
2．涡旋加入1 mg SA，涡旋混匀充分重悬，37℃摇床反应30 min；
3．涡旋加入0.1 mL 10 mg/mL EDC 溶液，37℃摇床反应4 h；
4．磁分离，留取上清测 SA 偶联率（BCA 法或其他）；
注：如需要封闭，请按步骤5进行；如无需封闭，请进入步骤6；
5．（可选步骤）加入封闭液彻底重悬磁珠，37℃摇床反应2 h；反应结束，磁分离弃上清，直接进行步骤7；
D．包被 SA 后清洗
6．步骤4结束后，加入1 mL 洗涤液1重悬磁珠，37℃摇床混匀30 min，磁分离弃上清；
7．加入1 mL 洗涤液2重悬磁珠，磁分离弃上清；重复此步骤3次；
8．加入磁珠保存液重悬磁珠，定容至实验所需浓度，保存于4℃备用。
注意事项
1．磁珠取用前应充分混匀，防止取用改变磁珠浓度，避免长时间超声对磁珠表面破坏；
2．磁珠使用前请进行磁分离并用纯水或所用缓冲溶液清洗2-3遍；
3．磁珠使用和保存过程中应避免冻融；
4．建议选用无菌低吸附移液吸头和离心管，避免因吸附造成磁珠或蛋白等损耗。
Aladdin Dextran Carboxyl Magnetic Beads (50nm) exhibit superparamagnetism, excellent suspension stability, good monodispersity, and strong resistance to nonspecific adsorption. The bead surface is rich in carboxyl groups, enabling covalent conjugation with proteins, antibodies, peptides, oligonucleotides, and other biomolecules. They are suitable for applications such as immunoassays, affinity purification, and cell sorting. The magnetic beads maintain excellent suspension stability in different buffer solutions, effectively ensuring reaction uniformity and consistent detection performance.
Product Information
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Reference Coupling Procedure, Using SA as an Example
A. Reagent Preparation
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B. Magnetic Bead Preparation and Washing
1. Thoroughly mix the magnetic beads. Transfer 10 mg of magnetic beads into a centrifuge tube, separate magnetically and discard the supernatant. Add 1 mL of coupling buffer to resuspend the beads, and mix by water-bath sonication for 20 s or vortexing. Wash once more with coupling buffer, then adjust the concentration to 10 mg/mL (resuspend to a final volume of 1 mL);
C. SA Coating and Magnetic Bead Blocking
2. Add 1 mg of SA while vortexing, vortex thoroughly to fully resuspend the beads, and incubate on a shaker at 37°C for 30 min;
3. Add 0.1 mL of 10 mg/mL EDC solution while vortexing, and incubate on a shaker at 37°C for 4 h;
4. Separate magnetically and retain the supernatant to determine the SA coupling efficiency (by BCA assay or another method);
Note: If blocking is required, proceed to Step 5; if blocking is not required, proceed to Step 6;
5. (Optional step) Add blocking solution to thoroughly resuspend the magnetic beads and incubate on a shaker at 37°C for 2 h. After the reaction, separate magnetically, discard the supernatant, and proceed directly to Step 7;
D. Washing After SA Coating
6. After Step 4, add 1 mL of Washing Solution 1 to resuspend the magnetic beads, mix on a shaker at 37°C for 30 min, then separate magnetically and discard the supernatant;
7. Add 1 mL of Washing Solution 2 to resuspend the magnetic beads, separate magnetically and discard the supernatant; repeat this step 3 times;
8. Add magnetic bead storage solution to resuspend the magnetic beads, adjust to the concentration required for the experiment, and store at 4°C until use.
Precautions
1. Thoroughly mix the magnetic beads before use to prevent changes in bead concentration during sampling, and avoid prolonged sonication to prevent damage to the bead surface;
2. Before use, magnetically separate the beads and wash them 2-3 times with purified water or the buffer solution to be used;
3. Avoid freeze-thaw cycles during use and storage of the magnetic beads;
4. It is recommended to use sterile low-binding pipette tips and centrifuge tubes to avoid loss of magnetic beads or proteins due to adsorption.
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