计算溶液所需的质量、体积或浓度。
BioReagent,用于细胞培养 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
室温。常规运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
平衡盐溶液(BSS)pH、渗透压模拟细胞生理环境,维持渗透压与酸碱平衡,供给细胞代谢所需无机盐与能量物质,保障体外细胞存活与基础代谢。主体为无机离子,可按需添加碳酸氢钠、葡萄糖、酚红、HEPES 等;BSS 衍生规格丰富,HBSS、EBSS、PBS、Earle’s 液、Hank’s 液均为常用品类。
阿拉丁 D-Hanks 平衡盐粉剂 (1×, 无 Ca²⁺、Mg²⁺, 含酚红) 不含 Ca²⁺、Mg²⁺,9.97 g/L,溶解后 pH 7.2–7.4;蒸馏水或去离子水溶解,过滤除菌,不可高压灭菌。仅科研用途,不用于临床诊断。
自备材料:
1、(无菌)蒸馏水或去离子水、稀盐酸、稀氢氧化钠
2、磁力搅拌器、烧杯、pH 计、过滤器
操作步骤(仅供参考):
1、取本品 1 L 规格粉剂 1 份(9.97 g),加入 1 L 蒸馏水或去离子水溶解,充分混匀后即可使用;如需无菌,采用过滤除菌,禁止高压灭菌。
注意事项:
1、在进行细胞培养过程中细胞的洗涤时,应注意无菌操作,避免被微生物污染。
2、本品不易析出沉淀;若出现少量沉淀,可置于温水浴中直至沉淀溶解后使用。如沉淀量较多,建议直接弃用。
3、本产品含营养成分,易变质,应注意密封保存。
4、为了您的安全和健康,请穿实验服并戴一次性手套操作。
Balanced Salt Solution (BSS) mimics the physiological pH and osmotic environment of cells. It maintains osmotic pressure and acid-base balance, supplies inorganic salts and energy substrates required for cellular metabolism, and supports cell survival and basal metabolism in vitro. It is mainly composed of inorganic ions and may be supplemented with sodium bicarbonate, glucose, phenol red, HEPES, etc. as needed. BSS is available in a wide range of formulations. HBSS, EBSS, PBS, Earle’s solution and Hank’s solution are commonly used types.
Aladdin D-Hanks Balanced Salt Powder (1×, without Ca²⁺, Mg²⁺, with phenol red). This product contains no Ca²⁺ or Mg²⁺. The formulation is 9.97 g/L, and the pH after dissolution ranges from 7.2 to 7.4. Dissolve in distilled or deionized water; sterilize by filtration. Do not autoclave. For research use only. Not for clinical diagnosis.
Materials Required but Not Provided:
1、(Sterile) distilled water or deionized water, dilute hydrochloric acid, dilute sodium hydroxide
2、Magnetic stirrer, beaker, pH meter, filter
Operating Procedure (For Reference Only):
1、Dissolve one pack of the product (1 L size, 9.97 g) in 1 L distilled or deionized water and mix thoroughly before use. Sterilize by filtration if required. Do not autoclave.
Precautions:
1、When washing cells during cell culture, aseptic techniques should be followed to avoid microbial contamination.
2、Precipitation rarely occurs. If a small amount of precipitate appears, warm the solution in a water bath until it dissolves before use. Discard the product if excessive precipitate forms.
3、This product contains nutritional components and is prone to deterioration. Ensure that it is stored in a sealed container.
4、For your safety and health, please wear a laboratory coat and disposable gloves during operation.
| pH | 7.2–7.4 |
|---|