计算溶液所需的质量、体积或浓度。
,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Denhardt溶液是一种阻断剂,通常用于减少检测试剂与Northern和Southern印迹膜的非特异性结合。该粉末可用于制备50倍浓缩液,可稀释至任何浓度,用于各种预杂交和杂交缓冲液。
应用
Denhardt溶液已被用于:作为杂交缓冲液的组分,用于脑切片的杂交用于原位杂交过氧化物酶-(抗过氧化物酶法-全标本包埋)(ISHPAP-WM)过程用于杂交混合物的制备适合用于Southern和Northern印迹膜的封闭缓冲液。
适用性
原理
制备说明
制备50倍浓缩液,将全部内容物溶解在5 mL分子生物级水中。在-20°C下可储存长达两年。
外形
重悬
组分
每管Denhardt粉末含有50mg牛血清白蛋白(BSA)、50mg Ficoll和50mg聚乙烯吡咯烷酮(PVP)。
Denhardt′s Solution is a blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. This powder can be used to make a 50x concentrate that can be diluted to any concentration for use in a variety of pre-hybridization and hybridization buffers.Denhardt′s Solution has been used:as a component in hybridization buffer for the hybridization of brain sectionsinin-situhybridization peroxidase-(antiperoxidase method-whole mounts) (ISHPAP-WM) processin the preparation of hybridization mixtureSuitable as a blocking Buffer for Southern and Northern blot membranes.