计算溶液所需的质量、体积或浓度。
,适用于对基线干扰要求严格的色谱和分析工作流程。
室温。常规运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Description
Duolink®proximity ligation assay(PLA®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.Follow theDuolink®In Situ Brightfield Protocol to use this product.Visit ourDuolink®PLA Resource Centerfor information on how to run a Duolink®experiment, applications, troubleshooting, and more.To perform a complete Duolink®in situ experiment you will need two primary antibodies(PLA, IHC, ICC or IF validated)that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink®PLA probes. Analysis is carried out using HRP for brightfield detection.SpecificityDuolink®In SituWash Buffer, Brightfield is used to wash specimen slides during Duolink®In Situ Brightfield procedures. See datasheet for more details.Application NoteTwo primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC), or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink®in situreagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.Let us do the work for you, learn more about ourCustom Service Programto accelerate your Duolink®projectsView full Duolink®product list