DL 550 Sulfhydryl-Reactive Dye is a maleimide-activated derivative of high-performance DL 550 used to fluorescently label sulfhydryl-containing peptides, proteins and other biomolecular probes.
Features of DL 550 Maleimide:
• Specific—maleimide-activated dye labels proteins and other molecules at reduced sulfhydryls (-SH) • Efficient labeling methods—well-characterized chemistry and optimized protocols provide for reliable, high-quality labeling • Optimized antibody labeling procedure—complete protocol for IgG reduction and labeling and calculating the labeling efficiency
Applications: • Antibody labeling for immunofluorescence applications, including immunocytochemistry (ICC), immunohistochemistry (IHC), Western blotting and ELISA assay • Target macromolecule labeling for in vitro and in vivo fluorescent detection strategies
DL 550 Sulfhydryl-Reactive Dye is activated with a maleic acid imide (maleimide) moiety to form a reactive alkylation reagent. Labeling occurs through reaction of the maleimide-activated dye with reduced sulfhydryl groups (-SH) to form stable thioether bonds. Maleimides are specific for sulfhydryl groups between pH 6.5-7.5. Learn more aboutmaleimide chemistry.