计算溶液所需的质量、体积或浓度。
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,-80℃储存。特低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 1 篇同行评审文献引用。
概述
Dencichin is a non-protein amino acid originally extracted from Panax notoginseng , and can inhibit HIF-prolyl hydroxylase-2 (PHD-2) activity.
In Vitro
Dencichin (β-ODAP, 10 μM, 50 μM, 100 μM and 200 μM) increases HRE expression by 1.3±0.09, 2.5±0.07, 4.2±0.15 and 1.3±0.07 fold respectively compared to control. Dencichin has intermolecular interactions with PHD-2. Dencichin (10 μM, 100 μM, 1 mM) significantly inhibits cell proliferation and extracellular matrix (ECM) proteins accumulation of HBZY-1 cells, and reduces the secretion of collagen I (Col I), collagen IV (Col IV), and fibronectin (FN). MCE has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Dencichin improves metabolism disorder in diabetic nephropathy (DN) secondary to type II diabetes mellitus (DM) model. Dencichin (80, 160 mg/kg/day, p.o.) significantly prevents the up-regulation of TCH, TG, LDL, and HbAlc and the down-regulation of HDL in DN rats induced by STZ injection. Dencichin also attenuates renal injury induced in the DN secondary to type II DM model. Dencichin alleviates pancreas damage in the STZ-induced DN model. Dencichin regulates protein expression in the TGF-β/Smad signalling pathway in STZ-induced DN models. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Assay
Cells are cultured in 6-well plates with glucose (LG) or high glucose control (HG) and then treated with valsartan (1.0 × 10 -3 M) or Dencichin (1.0 × 10 -5 M, 1.0 × 10 -4 M and 1.0 × 10 -3 M) for 24 h before analysing the activation of FN, Col I, and Col IV expression by ELISA and analysing MMP-9 and TIMP-1 expression by immunofluorescence. Each experiment is repeated at least three times throughout the study. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal administration
Male SD rats weighted approximately 200 ± 20 g (eight weeks old) are kept at 23 ± 2°C. The rats are randomLy allotted into two groups: normal group (n = 16) and diabetic nephropathy (DN) group (n = 60). The normal rats fed with normal diet and vehicle fall into the normal control group (NC, n = 8) and the normal rats fed with normal diet and Dencichin fall into the Dencichin control group (De C, n = 8). The rats in the diabetic group are fed the high-sugar-high-fat diet (composition: Common breeding material 54.6%, lard oil 16.9%, sucrose 14%, casein 10.2%, gunk 2.1%, maltodextrin 2.2%). Five weeks later, DN is induced by administering of 40 mg/kg STZ though intraperitoneal injection, and the normal rats are treated with vehiclecitrate buffer (0.1 M, pH 4.2). Next, the DN rats (n = 32) are assigned into four groups and oral administered with metformin hydrochloride (a positive control) at 80 mg/kg/day, (DN+Met, n = 8), which is dissolve into distilled water to make a 2 mg/mL solution before use; vehicle control for the DN control group (DN, n = 8), or Dencichin. The Dencichin group is divided into a high Dencichin group ( 160 mg/kg/day ; DN+De H, n = 8) and a low Dencichin group ( 80 mg/kg/day ; DN+De L, n = 8), which are dissolved into distilled water to make a 5 mg/mL and 2.5 mg/mL solution before use and oral administered at the dosage once per day. Blood glucose is measured each month. 8 w after administering with Dencichin, the rats are killed . aladdin has not independently confirmed the accuracy of these methods. They are for reference only.
IC50& Target:PHD-2
规格
别名
丹茜奇尼
规格或纯度
10mM in DMSO
英文名称
Dencichine
生化机理
Dencichin 最初是从三七中提取的一种非蛋白氨基酸，可抑制 HIF-脯氨酰羟化酶-2（PHD-2）的活性。
储存条件
避光,-80℃储存
运输条件
特低温运输
作用类型
抑制剂
名称和识别符
分子类型
小分子
Isomeric SMILES
C([C@@H](C(=O)O)N)NC(=O)C(=O)O
关联CAS
PubChem CID
MeSH Entry Terms
(2-amino-2-carboxymethyl)-L-oxamic acid;2-oxalylamino-3-aminopropionic acid;3-amino-N-(carboxycarbonyl)-DL-alanine;3-amino-N-(carboxycarbonyl)alanine;3-oxalylamino-2-aminopropionic acid;beta-N-oxalylamino-L-alanine;beta-N-oxalylaminoalanine;beta-N-oxalyla
分子量
176.13
技术文档
高级数据
安全和危险性（GHS）
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
此产品的引用文献
溶液计算器
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