金属增强 DAB 底物在特殊配方中使用氯化钴和氯化镍，在辣根过氧化物酶（HRP）存在下产生深褐色/黑色沉淀。3, 3′二氨基联苯胺四盐酸盐（DAB）是一种应该安全使用的致癌物质。它是 HRP（辣根过氧化物酶）的主要敏感底物之一。DAB 呈棕色或琥珀色，由于过氧化氢和过氧化物酶的存在，变得不溶，并在抗原-抗体位点沉淀。

The metal enhanced DAB Substrate utilizes cobalt chloride and nickel chloride in a special formulation to produce a dark brown/black precipitate in the presence of horseradish peroxidase (HRP).
3, 3′ diaminobenzidine tetrahydrochloride (DAB) is a carcinogen that should be used safely. It is one of the major sensitive substrates for HRP (horseradish peroxidase). DAB is brown or amber in color and becomes insoluble and precipitates over the antigen-antibody site due to the presence of hydrogen peroxide and peroxidase.

Application:

DAB Substrate is a color sustrate for peroxidases and is used in a variety of applications:Immunohistocytochemistry
In situ hybridization
Western blot
Dot blot

DAB substrate has been used in immunofluorescence and western blot.

规格

产品名称

DAB 底物

规格或纯度

dark brown, /black precipitate; Visually evaluated

分子类型

生物试剂/缓冲液

储存与运输

物理形态

固体

浓度

dark brown, /black precipitate; Visually evaluated

储存条件

-20°C储存

运输条件

超低温运输

高级数据

质检证书(CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

技术文档和文章

过氧化物酶底物检测系统

目录：技术文章

探索主题:

HRP 免疫检测试剂和底物酶抑制剂和酶底物过氧化物酶 TMB DAB ABTS 显色底物和化学发光底物

Peroxidase Substrate Detection System

目录：技术文章

探索主题:

HRP Immunoassay reagents and substrates TMB DAB ABTS Peroxidase Enzyme inhibitors and substrates Chromogenic substrates and chemiluminescence substrates

发色底物法的原理、类型与检测方法

目录：技术文章

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Chromogenic Substrate Chromogenic substrate assay color reaction absorbance detection endpoint method kinetic method enzyme activity assay

Principles, Types, and Detection Methods of Chromogenic Substrate Assays

目录：技术文章

探索主题:

酶活测定显色反应发色底物法发色底物吸光度检测终点法动态检测法

溶液计算器