Evo312 is a dose-dependent inhibitor of protein kinase CβⅠ ( PKCβⅠ ) ( IC 50 is 117.34 nM). Evo312 induces PANC-GR (acquired gemcitabine-resistant PC cells) cell cycle arrest and apoptosis by inhibiting PKCβ1 protein expression. Evo312 has antiproliferative effects in pancreatic cancer cells PANC-1 and PANC-GR cells with IC 50 of 0.08 μM and 0.07 μM, and in human normal pancreatic ductal epithelial cells HPDE6-c7 with IC 50 of 2.95 μM. Evo312 exhibits antitumor activity in a PANC-GR cell transplantation mouse model.