计算溶液所需的质量、体积或浓度。
BioReagent,for ELISA 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
ELISA封闭液(5%BSA)主要由磷酸氢二盐、磷酸二氢盐、BSA、防腐剂等组成,主要用于ELISA实验中对96孔板或其他固相载体进行封闭处理。该试剂仅用于科研领域,不适用于临床诊断或其他用途。
操作步骤(仅供参考)
1. 按要求包被96孔板,用ELISA 洗涤液洗涤。
2. ELISA 封闭液(5% BSA)对96孔板或其他固相载体进行封闭处理,一般 37℃孵育1-2 h。
3. 封闭完成后洗涤3次,将包被好的96孔板拍干、密封,4℃保存。
4. 根据实验需求进行一抗、二抗等步骤。
5. 加入配制好的显色底物,37°反应10 min左右。
6. 加入 ELISA 终止液终止反应。
注意事项
1. 注意密闭保存,一旦开封保质期就会大大缩短。
2. ELISA 封闭液(5% BSA)浓度较高,可用PBS稀释后使用。
3. 该试剂属于营养物质,注意无菌操作,尽量避免污染。
4. 试剂开封后请尽快使用,以防影响后续实验效果。
ELISA Blocking Buffer (5% BSA) is mainly composed of disodium hydrogen phosphate, sodium dihydrogen phosphate, BSA, and preservatives. It is designed for blocking 96‑well plates or other solid-phase carriers in ELISA assays. This product is intended for research use only and is not suitable for clinical diagnosis or other purposes.
Procedure (for reference only)
1. Coat the 96‑well plate as required and wash with ELISA washing buffer.
2. Block the coated plate (or other solid-phase carrier) with ELISA Blocking Buffer (5% BSA). Typically, incubate at 37 °C for 1–2 h.
3. After blocking, wash the plate three times. Tap the plate dry, seal it, and store at 4 °C.
4. Proceed with primary antibody, secondary antibody, and subsequent steps according to experimental needs.
5. Add the prepared chromogenic substrate and incubate at 37 °C for about 10 minutes.
6. Terminate the reaction by adding ELISA stop solution.
Precautions
1. Store tightly sealed. Once opened, the shelf life will be significantly shortened.
2. ELISA Blocking Buffer (5% BSA) is supplied at a high concentration and can be diluted with PBS before use if needed.
3. Because the reagent contains nutrients, maintain aseptic handling to avoid contamination.
4. Use promptly after opening to prevent any impact on subsequent experimental performance.
通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | E1508756 | |
| 分析证书 | E1508756 | |
| 分析证书 | E1508756 | |
| 分析证书 | E1508756 | |
| 分析证书 | E1508756 | |
| 分析证书 | E1508756 |