嗜酸性粒细胞 (eosinophil, E) 是白细胞的一种，占白细胞总数的 0.5%～5%。嗜酸性粒细胞稀释液 (Eosinophil Dilution) 作用原理是血液经适量的 Eosinophil Dilution 稀释，嗜酸性粒细胞在含有石楠红 B 中被染成红色，而红细胞及其他白细胞破裂或溶解，有时少量并未被破坏，但不会被伊红着色。充入计数池内，在显微镜下计数一定体积内嗜酸性粒细胞数量，换算求出每升血液中嗜酸性粒细胞的数量。该嗜酸性粒细胞稀释液仅用于科研领域，不用于临床诊断。
操作步骤(仅供参考)：
1、取小号试管，加入 Eosinophil Dilution。
2、用洁净干燥微量吸管取末梢血，加至 Eosinophil Dilution 中，充分混匀。
3、待红细胞溶解后，充入计数板内两侧池内，注意产生气泡或外溢，室温静置 3～5min，嗜酸性粒细胞沉淀。
4、置于显微镜低倍镜 (必要时可用高倍镜) 下依次计数两个计数池中 10 个大方格内嗜酸性粒细胞数。
计算：嗜酸性粒细胞数/L=10 个中方格内嗜酸性粒细胞×20×10⁶/L
注意事项：
1、血液稀释后，应在 1h 内计数完毕，否则嗜酸性粒细胞会逐渐被破坏。
2、充池前应充分混匀，充池时注意不宜用力过大。
3、注意与中性粒细胞区别，后者一般不着色，偶有浅红色，颗粒较小。
4、嗜酸性粒细胞较少时，可增加计数面积。
5、为了您的安全和健康，请穿实验服并戴一次性手套操作。
Eosinophils (E) are a type of leukocyte, accounting for 0.5%–5% of the total leukocyte count. The working principle of Eosinophil Dilution is as follows: blood is diluted with an appropriate volume of Eosinophil Dilution, and eosinophils are stained red in the presence of Phloxine B, while red blood cells and other leukocytes rupture or lyse. Occasionally, a small number of them remain intact but will not be stained by eosin. The diluted blood is loaded into the counting chamber, and the number of eosinophils in a defined volume is counted under a microscope to calculate the eosinophil concentration per liter of blood. This Eosinophil Dilution is for research use only and not intended for clinical diagnosis.
Operating Procedures (For Reference Only):
1. Take a small test tube and add Eosinophil Dilution to it.
2. Use a clean, dry micropipette to draw peripheral blood, add it to the Eosinophil Dilution, and mix thoroughly.
3. After the red blood cells have lysed, load the diluted blood into both sides of the counting chamber, taking care to avoid bubble formation or overflow. Allow to stand at room temperature for 3–5 minutes to let eosinophils sediment.
4. Place the counting chamber under a low-power microscope (a high-power microscope may be used if necessary) and count the number of eosinophils in 10 large squares across the two counting chambers in sequence.
Calculation: Eosinophil count/L = Number of eosinophils in 10 medium squares × 20 × 10⁶/L
Precautions:
1. Counting should be completed within 1 hour after blood dilution; otherwise, eosinophils will be gradually damaged.
2. Mix the diluted blood thoroughly before loading it into the counting chamber, and avoid applying excessive force during the loading process.
3. Distinguish eosinophils from neutrophils; the latter are generally unstained or occasionally pale red with smaller granules.
4. If the number of eosinophils is low, the counting area can be increased.
5. For your safety and health, wear a lab coat and disposable gloves during operation.
ZJ26F0333053
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