计算溶液所需的质量、体积或浓度。
BioReagent,生物染色剂,用于显微镜 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
嗜酸性粒细胞是炎症细胞成分之一,多在变态反应性疾病中发挥作用,嗜酸性粒细胞质中含次级颗粒,上述颗粒中主要含有碱性蛋白,如嗜酸性粒细胞阳离子、嗜酸性粒细胞衍生神经毒素、嗜酸性粒细胞过氧化物酶等。
嗜酸性粒细胞染色液由Carbol2R染色液、苏木素染色液组成,嗜酸性粒细胞的胞质呈粉红色,核呈蓝色。该试剂仅用于科研领域,不适用于临床诊断或其他用途。
产品组分及储存条件:
| E1511531 | Component | 2×100mL | Storage |
| E1511531A | Carbol 2R 染色液 | 100mL | RT. Store in the dark. |
| E1511531B | 苏木素染色液 | 100mL | RT |
操作步骤(仅供参考):
1. 组织固定 (推荐选择10%中性福尔马林固定液),常规脱水包埋,常规脱蜡至水。
2、入Carbol2R染色液,浸染15~20min,蒸馏水清洗。
3、入苏木素染色液,浸染3~8min,蒸馏水清洗。
4、系列乙醇脱水,常规透明,中性树胶封片。
染色结果:
嗜酸性粒细胞胞质:粉红色
细胞核:蓝色
注意事项:
1、分色不佳时,可用0.5%盐酸乙醇分化,以去除背景。
2、亦可用于细胞涂片染色,但染色时间应相应缩短,固定采用4%多聚甲醛溶液。
3、试剂开封后请尽快使用,以防影响后续实验效果。
4、为了您的安全和健康,请穿实验服并戴一次性手套操作。
Eosinophils are one type of inflammatory cellular component and mostly function in allergic diseases. The cytoplasm of eosinophils contains secondary granules, which mainly comprise basic proteins, such as eosinophil cationic protein, eosinophil-derived neurotoxin, and eosinophil peroxidase.
Eosinophil Staining Solution consists of Carbol 2R Staining Solution and Hematoxylin Staining Solution. The cytoplasm of eosinophils is stained pink, and the nucleus is stained blue. This reagent is for research use only and not for clinical diagnosis or other purposes.
Product Components and Storage Conditions:
| E1511531 | Component | 2×100mL | Storage |
| E1511531A | Carbol 2R Stain | 100mL | RT. Store in the dark. |
| E1511531B | Hematoxylin Stain | 100mL | RT |
Protocol (For Reference Only):
1. Tissue fixation (10% neutral formalin fixative is recommended), followed by routine dehydration, embedding, and dewaxing to water.
2. Stain in Carbol 2R Stain for 15–20 min, then rinse with distilled water.
3. Stain in Hematoxylin Stain for 3–8 min, then rinse with distilled water.
4. Dehydrate in graded ethanol, clear routinely, and mount with neutral balsam.
Staining Results:
Cytoplasm of eosinophils: Pink
Nuclei: Blue
Precautions:
1. If differentiation is unsatisfactory, 0.5% hydrochloric acid alcohol may be used for differentiation to remove background.
2. Can also be used for staining cell smears, but the staining time should be shortened accordingly; fix with 4% paraformaldehyde solution.
3. Please use the reagent as soon as possible after opening to avoid affecting experimental results.
4. For your safety and health, please wear a lab coat and disposable gloves during operation.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | E1511531 |
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