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外切-β-1,4-葡聚糖酶（又称纤维二糖水解酶，CBH，EC 3.2.1.91）广泛存在于细菌、真菌及动物体内，是纤维素酶系的重要组分之一。该酶催化底物4-硝基苯-β-D-纤维二糖苷水解，释放对硝基苯酚（PNP）。PNP在405 nm处具有最大吸收峰，通过测定该波长下吸光度，即可定量计算外切-β-1,4-葡聚糖酶的酶活力。
Exo-β-1,4-glucanase, also known as cellobiohydrolase (CBH, EC 3.2.1.91), widely exists in bacteria, fungi and animals, and serves as an important component of the cellulase system. This enzyme catalyzes the hydrolysis of the substrate 4-nitrophenyl-β-D-cellobioside to release p-nitrophenol (PNP). PNP has a maximum absorption peak at 405 nm, and the enzyme activity of exo-β-1,4-glucanase can be quantitatively calculated by measuring the absorbance at this wavelength.
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