本培养基专用转基因组培预培养、侵染和共培养。本培养基并非传统MS培养基，而是在MS基础上改善渗透压等条件，旨在减少外植体玻璃化及褐化。同时，降低了农杆菌侵染引起的生理胁迫，大大提高了后续分化的效率，缩短转化周期。
组分&储存
| 组件货号 | 组件名称 | 物理外观 | 8L | 储存温度 |
| E1529435A | 组分A | 液体 | 160ml | 2-8℃ |
| E1529435B | 组分B | 液体 | 80ml | 2-8℃ |
| E1529435C | 组分C | 液体 | 80ml | 2-8℃ |
| E1529435D | 有机物GBR5 | 液体 | 8 x1ml | 2-8℃ |
| E1529435E | AS | 液体 | 8 x1ml | 2-8℃ |
使用说明
1、在烧杯中加入4ml A 液、2ml B 液、2ml C 液，6g 糖，约1.6‑1.7g 琼脂(琼脂按自己购买产品说明书加入量加入，也可调节好pH后加入) 定容至 200ml 即可配置为侵染专用固体培养基，不加琼脂即为侵染液(培养基和侵染液体积可自行按比例放大缩小)。
2、混合好后加入约 70μl 0.5 M NaOH 调节 pH 到 5.8。 注:水质不同所加入的 NaOH 可能有区别，可自行调节；
3、120℃，灭菌 20min。
4、待培养基温度降至 45‑50℃ 时，加入 GBR5 200μl 及对应物种的激素。AS 可自行选择是否添加(侵染液中必须添加，每 200ml 添加 200μl)。
注：本产品不含蔗糖和琼脂，如实验需添加上述成分，请自行准备或联系本公司购买；实验中所需激素（依实验物种而定）亦请自行准备或联系本公司购买。
注意事项
1、试剂开封后请尽快使用，以防影响后续实验效果。
2、为了您的安全和健康，请穿实验服并戴一次性手套操作。
3、本品为实验室科研用试剂，仅供科研实验使用，不可用于人体、临床诊断、食品及家庭用途等。
This medium is specially used for the pre‑culture, infection and co‑cultivation stages of transgenic plant tissue‑culture. Unlike the traditional MS medium, it is modified from MS basal medium with optimized osmotic pressure and other conditions, so as to reduce vitrification and browning of explants. Meanwhile, it alleviates the physiological stress induced by Agrobacterium infection, greatly improves the subsequent differentiation efficiency and shortens the transformation cycle.
Contents & Storage
| Component No. | Component Name | Appearance | 8L | Storage |
| E1529435A | Component A | Liquid | 160ml | 2-8℃ |
| E1529435B | Component B | Liquid | 80ml | 2-8℃ |
| E1529435C | Component C | Liquid | 80ml | 2-8℃ |
| E1529435D | Organic matter GBR5 | Liquid | 8 x1ml | 2-8℃ |
| E1529435E | AS | Liquid | 8 x1ml | 2-8℃ |
Instructions for Use
1. Add 4 mL of Solution A, 2 mL of Solution B, 2 mL of Solution C, 6 g of sugar, and approximately 1.6‑1.7 g of agar into a beaker (the agar addition amount shall follow the manual of your purchased‑agar product; agar can also be added after pH adjustment). Make up the final volume to 200 mL to prepare the solid medium for infection. Without agar, the mixture serves as infection solution. The volumes of medium and infection solution can be scaled up or down proportionally.
2. Add approximately 70 μL of 0.5 M NaOH to adjust the pH to 5.8 after mixing. Note: The required dosage of NaOH may vary with water quality and can be adjusted accordingly.
3. Sterilize at 120 °C for 20 min.
4. When the medium cools down to 45‑50 °C, add 200 μL of GBR5 and hormones specific to the experimental species. The addition of AS is optional for medium preparation (AS must be added into infection solution at a dosage of 200 μL per 200 mL).
Note: This product contains no sucrose or agar. If these components are required for your experiment, please prepare them separately or purchase them from our company. Hormones required for the experiment (species‑dependent) also need to be prepared separately or purchased from our company.
Precautions
1. Use the reagent promptly once opened to avoid adverse impacts on subsequent experiments.
2. Wear a lab coat and disposable gloves during operation for your safety and health.
3.This is a laboratory‑grade research‑only reagent. It is intended solely for scientific‑research purposes and shall not be used for human‑related applications, clinical diagnosis, food processing or household use.
危险品化学品经营许可证（带存储）