计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
本培养基专用转基因组培预培养、侵染和共培养。本培养基并非传统MS培养基,而是在MS基础上改善渗透压等条件,旨在减少外植体玻璃化及褐化。同时,降低了农杆菌侵染引起的生理胁迫,大大提高了后续分化的效率,缩短转化周期。
组分&储存
| 组件货号 | 组件名称 | 物理外观 | 8L | 储存温度 |
| E1529436A | 组分A | 液体 | 160ml | 2-8℃ |
| E1529436B | 组分B | 液体 | 80ml | 2-8℃ |
| E1529436C | 组分C | 液体 | 80ml | 2-8℃ |
| E1529436D | 组分D | 液体 | 8 x1ml | 2-8℃ |
| E1529436E | 有机物GBR5 | 液体 | 8 x1ml | 2-8℃ |
使用说明
1、在烧杯中加入 4ml 组分A 、2ml 组分 B、2ml 组分 C,6g 糖,约 1.6‑1.7g 琼脂 (琼脂按自己购买产品说明书加入量加入,也可调节好 pH 后加入) 定容至 200ml 即可配置为侵染专用固体培养基,不加琼脂即为侵染液 (培养基和侵染液体积可自行按比例放大缩小)。
2、混合好后加入约 70μl 0.5 M NaOH 调节 pH 到 5.8; 注:水质不同所加入的 NaOH 可能有区别,可自行调节。
3、120℃,灭菌 20min。
4、待培养基温度降至 45‑50℃ 时,加入有机物 GBR5 200 μl 和组分 D 260μl。
注意事项
1、试剂开封后请尽快使用,以防影响后续实验效果。
2、为了您的安全和健康,请穿实验服并戴一次性手套操作。
3、本品为实验室科研用试剂,仅供科研实验使用,不可用于人体、临床诊断、食品及家庭用途等。
This medium is specially used for the pre‑culture, infection and co‑cultivation stages of transgenic plant tissue‑culture. Unlike the traditional MS medium, it is modified from MS basal medium with optimized osmotic pressure and other conditions, so as to reduce vitrification and browning of explants. Meanwhile, it alleviates the physiological stress induced by Agrobacterium infection, greatly improves the subsequent differentiation efficiency and shortens the transformation cycle.
Contents & Storage
| Component No. | Component Name | Appearance | 8L | Storage |
| E1529436A | Component A | Liquid | 160ml | 2-8℃ |
| E1529436B | Component B | Liquid | 80ml | 2-8℃ |
| E1529436C | Component C | Liquid | 80ml | 2-8℃ |
| E1529436D | Component D | Liquid | 8 x1ml | 2-8℃ |
| E1529436E | Organic matter GBR5 | Liquid | 8 x1ml | 2-8℃ |
Instructions for Use
1. In a beaker, add 4 mL of Component A, 2 mL of Component B, 2 mL of Component C, 6 g of sugar, and approximately 1.6‑1.7 g of agar (add agar according to the manufacturer’s instructions of your purchased product; agar may also be added after pH adjustment). Bring the total volume to 200 mL to prepare solid medium for infection. Omit agar to obtain infection solution. The volume of medium and infection solution can be scaled up or down proportionally as required.
2. After mixing, add approximately 70 μL of 0.5 M NaOH to adjust the pH to 5.8. Note: The required volume of NaOH may vary depending on water quality; adjust accordingly.
3. Sterilize at 120 °C for 20 min.
4. When the medium cools down to 45‑50 °C, add 200 μL of Organic matter GBR5 and 260 μL of Component D.
Precautions
1. Use the reagent promptly once opened to avoid adverse impacts on subsequent experiments.
2. Wear a lab coat and disposable gloves during operation for your safety and health.
3. This is a laboratory‑grade research‑only reagent. It is intended solely for scientific‑research purposes and shall not be used for human‑related applications, clinical diagnosis, food processing or household use.
| 组件货号 | 组件名称 | 物理外观 | 8L | 储存温度 |
| E1529436A | 组分A | 液体 | 160ml | 2-8℃ |
| E1529436B | 组分B | 液体 | 80ml | 2-8℃ |
| E1529436C | 组分C | 液体 | 80ml | 2-8℃ |
| E1529436D | 组分D | 液体 | 8 x1ml | 2-8℃ |
| E1529436E | 有机物GBR5 | 液体 | 8 x1ml | 2-8℃ |