计算溶液所需的质量、体积或浓度。
BioReagent,用于蛋白分析,适用于电泳 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
本产品适用于 EMSA(Electrophoretic Mobility Shift Assay,凝胶迁移实验,又称 Gel-Shift Assay)。该技术通过分析目的蛋白与特定 DNA 序列的结合情况,评估细胞内转录因子的激活水平。本缓冲液专用于细胞核蛋白或纯化转录因子与特定双链寡核苷酸完成结合反应后的样品上样。
产品特点:
无色配方(不含溴酚蓝):专为荧光标记探针、同位素或化学发光检测体系设计,避免染料颜色对成像信号的干扰,确保检测结果的高信噪比。
使用说明:
按照每 3 μL 样品加入 1μL 上样缓冲液 (4×)的比例混合,混匀后立即上样。
注意事项:
1.需自备 EMSA 相关的所有其它试剂。
2.仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
3.为了您的安全和健康,请穿戴好个人防护装备和服装进行操作。
This product is suitable for EMSA (Electrophoretic Mobility Shift Assay, also known as gel-shift assay). This technique analyzes the binding between target proteins and specific DNA sequences to evaluate the activation level of transcription factors in cells. This buffer is designed for sample loading following binding reactions between nuclear proteins or purified transcription factors and specific double-stranded oligonucleotides.
Product Feature:
• Colorless formulation (bromophenol blue free): Optimized for fluorescently labeled probes, radioactive or chemiluminescent detection systems. It avoids signal interference from dye color during imaging and ensures high signal-to-noise ratio.
Instructions for Use:
Mix at a ratio of 1 μL of loading buffer (4×) per 3 μL of sample. After mixing thoroughly, load immediately.
Precautions:
1.All other reagents required for EMSA must be provided by the user.
2.This product is for research use by professionals only. It must not be used for clinical diagnosis or treatment, for food or drugs, or stored in ordinary residential buildings.
3.For your safety and health, please wear appropriate personal protective equipment and clothing during operation.
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 Suitable for electrophoresis 级别指南 → 查看 BioReagent 级别指南 → 查看 for Protein analysis 级别指南 →