计算溶液所需的质量、体积或浓度。
BioReagent,用于蛋白分析,适用于电泳 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
本产品适用于 EMSA(凝胶迁移实验,Gel-Shift Assay),用于研究目的蛋白与特定 DNA 序列的结合情况,进而评估细胞内转录因子的激活水平。本品专用于细胞核蛋白或纯化转录因子与特定双链寡核苷酸完成结合反应后的样品上样。
特别说明与替代方案:
本产品含有溴酚蓝作为电泳指示剂。在某些特定的 EMSA 体系中,溴酚蓝可能会对目的转录因子与双链寡核苷酸的结合反应产生一定干扰(如影响结合复合物的迁移或信号检测)。
若您的实验对染料敏感,建议选用不含溴酚蓝的替代产品:EMSA/Gel-Shift 上样缓冲液 (4×, 无色)(阿拉丁货号:E1547550)。
使用说明:
按照每 3 μL 样品加入 1μL 上样缓冲液 (4×)的比例混合,混匀后立即上样。
注意事项:
1.需自备 EMSA 相关的所有其它试剂。
2.仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
3.为了您的安全和健康,请穿戴好个人防护装备和服装进行操作。
This product is suitable for EMSA (Gel-Shift Assay) to study the binding between target proteins and specific DNA sequences, so as to evaluate the activation level of transcription factors in cells. It is designed for sample loading after binding reactions between nuclear proteins or purified transcription factors and specific double-stranded oligonucleotides.
Notes & Alternative Option:
This product contains bromophenol blue as an electrophoresis tracking dye. In certain EMSA systems, bromophenol blue may interfere with the binding reaction between the target transcription factor and double-stranded oligonucleotides (e.g., altering the migration of binding complexes or affecting signal detection).
If your assay is dye-sensitive, we recommend the bromophenol-blue-free alternative: EMSA/Gel-Shift Loading Buffer (4×, colorless) (Aladdin Cat. No.: E1547550).
Instructions for Use:
Mix at a ratio of 1 μL of loading buffer (4×) per 3 μL of sample. After mixing thoroughly, load immediately.
Precautions:
1.All other reagents required for EMSA must be provided by the user.
2.This product is for research use by professionals only. It must not be used for clinical diagnosis or treatment, for food or drugs, or stored in ordinary residential buildings.
3.For your safety and health, please wear appropriate personal protective equipment and clothing during operation.
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