计算溶液所需的质量、体积或浓度。
概述
电泳样品缓冲液是一种现成的还原性电泳样品缓冲液，含溴酚蓝，用于制备要在SDS-PAGE中分离的蛋白质样品。电泳样品缓冲液中包含溴酚蓝，作为跟踪染料，可在电泳过程中监控样品。2X电泳样品缓冲液包含DTT，该酶可破坏二硫键，以确保蛋白质在加载到凝胶上之前已完全变性。电泳样品缓冲液还包含甘油，TCEP，DTT，SDS，1.0M Tris-HCl pH 6.7和溴酚蓝。对于非还原SDS-PAGE，建议使用电泳样品缓冲液（非还原）。
电泳样品缓冲液（非还原性）是一种即用型非还原电泳样品缓冲液，含溴酚蓝，用于制备要在非还原性凝胶中分离的蛋白质样品。分析天然蛋白质的特性时，使用非还原SDS-PAGE（不沸腾和不还原剂）
产品规格与参数
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Electrophoresis Sample Buffer, is a ready-to-use reducing electrophoresis sample buffer solution with bromophenol blue for the preparation of protein samples to be separated in SDS-PAGE. Bromophenol Blue is included with Electrophoresis Sample Bufferas a tracking dye to monitor the sample during the electrophoretic run. Electrophoresis Sample Buffer, contains DTT (sc-29089), which disrupts disulfide bonds to ensure proteins are fully denatured before loading on the gel. Electrophoresis Sample Buffer also contains glycerol, TCEP, DTT, SDS, 1.0M Tris-HCl pH 6.7 and bromophenol blue. For non-reducing SDS-PAGE, Electrophoresis Sample Buffer (non-reducing) (sc-45085) is recommended.
Mix sample with an equal volume of Electrophoresis Sample Buffer, .Bring to 95° C for 10 minutes, cool to room temperature before loading. If particulate is present, centrifuge samples 5 minutes at 14k RPM in microcentrifuge, and load the gel.
Electrophoresis Sample Buffer is a ready-to-use reducing sample buffer for SDS-PAGE, acting on denatured proteins in the system.
Electrophoresis Sample Buffer, 2X (non-reducing) is a ready-to-use non-reducing electrophoresis sample buffer solution with bromophenol blue for the preparation of protein samples to be separated in non-reducing gels. Non-reducing SDS-PAGE (no boiling and no reducing agent) is used when the properties of native proteins are being analyzed.|Contains glycerol, 0.217 M Tris-HCl pH 6.8 and bromophenol blue.|For reducing SDS-PAGE, Electrophoresis Sample Buffer 2X (sc-24945) is recommended.
Electrophoresis Sample Buffer, 2X (non-reducing)is a ready-to-use, non-reducing sample buffer solution
Product specifications and parameters
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规格
英文名称
Electrophoresis Sample Buffer
储存条件
2-8°C储存
运输条件
低温运输
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
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溶液计算器
常见问题
本产品应如何储存？
请于2–8 °C条件下保存。需冷藏保存以维持规定的有效期。
本产品如何运输？
本产品采用湿冰冷藏运输。收货后请立即拆包，并按上述储存条件保存。
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产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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