F2302351计算溶液所需的质量、体积或浓度。
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存,避光。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
Product Characteristics
Effect Diluents, Animal-free are effective buffers free of any animal components. They can be used for the dilution of serum, plasma, blood, stool or urine samples, as well as the dilution of primary and secondary antibodies. Effect Diluents, Animal-free efficiently minimize matrix effects, cross-reactions and unspecific binding in immunoassays like ELISA, Western blotting, Immunohistochemistry, protein arrays and immuno-PCR.
The Effect Diluents, Animal-free are used alternatively to the standard sample or antibody dilution buffers:
In ELISA for the dilution of specimen and detection antibodies.
In Western Blotting for the dilution of primary and secondary antibodies.
In Protein arrays for the dilution of specimen and detection antibodies.
In immuno-PCR as a washing buffer.
Three versions of the diluent are offered: Low, Medium and High for optimal discrimination between specific and unspecific reaction and for minimizing strong interference effects e.g., by RF (rheumatoid factors), HAMAs (human-a-mouse Abs) or by endogenous components that bind and mask the analyte.
Composition & Properties
The Effect Diluents, Animal free contain no animal components and are free of phosphates.
Working Procedure
1.Mix thoroughly prior to use.
2.Dilution recommendations
a.Dilute antibodies according to the instruction of the antibody
b.Dilution of the specimen is recommended at 1:2 or higher
Tips & Tricks
Effect Diluents must not be considered as blocking buffers. Recommended blocking buffers are: Synthetic Blocking Buffer, ELISA (cat. no. S494401), Synthetic Blocking Buffer, Blotting (cat. no. S494457) and WellChampion (cat. no. W494467) for plate blocking and stabilization (preparation of pre-coated plates).
Complex sample matrices, such as serum and plasma, may contain interfering factors that affect the ability of the assay to accurately quantify the target analyte. Strong interferences are often caused by RFs and HAMAs. This matrix effect can cause high background in the negative control or false negatives in the sample measurement. To reduce this effect the samples can be diluted in the Effect Diluents, Animalfree.
Handling & Storage
Store solution 2-8°C or -15 to -30°C (tolerates freezing and thawing cycles)
规格
别名
效果稀释剂培养基，无动物成分
规格或纯度
Ready-to-use (RTU)
英文名称
Effect Diluent Medium, Animal-free
储存条件
2-8°C储存,避光
运输条件
低温运输
名称和识别符
分子类型
培养基
技术文档
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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