概述规格名称和识别符技术文档高级数据质检证书（CoA,COO,BSE/TSE 和分析图谱) 技术文档和文章溶液计算器

🧪

为什么选择此级别

，适用于对基线干扰要求严格的色谱和分析工作流程。

🌡

储存与运输

-20°C储存,避免反复冻融。超低温运输。请查阅批次 COA 获取详细规格。

📋

质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

📚

文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

概述

本产品是快速逆转录试剂混合液，5×EasyQuick RT MasterMix中包含EasyQuick RT逆转酶、RNase Inhibitor、Random 6 mers、Oligo dT Primer、dNTP、EQ-RT Buffer 等从RNA模板逆转录成cDNA第一链所需的全部试剂。该产品的逆转录效率高，可对少量RNA模板进行良好的逆转录反应，15分钟即可完成荧光定量模板cDNA第一链合成。本试剂盒操作十分方便快捷，仅需加入RNA模板和水即可进行逆转录反应，特别适合于高通量检测。

E665905	Component	200 T	Storage
E665905A	5×EasyQuick RT MasterMix	400 μL	-20℃. Avoid freeze/thaw cycle.
E665905B	RNase-Free Water	2×1 mL	-20℃. Avoid freeze/thaw cycle.

产品特点

1．简便：即用型逆转录Mix，只需加入RNA模板和水，便可进行反应。

2．快速：15分钟完成cDNA第一链合成。

3．反转录效率高：反转录效率高于90%。

4．灵敏度高：pg级模板也可以得到高质量的cDNA。

5．通读复杂模板：GC含量高和二级结构复杂模板。

注意事项

1．在操作过程中应避免RNase污染，防止RNA降解或实验中的交叉污染，建议操作人员带口罩和一次性手套并经常更换手套，使用专门的仪器和耗材。

2．逆转录体系配制在冰上进行操作，防止RNA发生降解。试剂盒的MasterMix使用后尽快置于-20℃保存，并尽量避免反复冻融。

3．10 μl反应体系可最大使用1 μg总RNA，如果模板RNA的量大于1 μg，请按比例扩大反应体系。

4．对于二级结构复杂的RNA模板，建议在操作步骤之前，将模板RNA在65℃孵育5 min 立刻置于冰上，短暂离心后进行下一步操作。

操作步骤

1．将模板RNA在冰上解冻；将试剂盒组分在室温解冻后立刻置于冰上，使用前将每种溶液涡旋振荡混匀，并经短暂离心后使用。

2．根据以下表格配制反应体系（反应液配制请在冰上进行），涡旋震荡混匀，短暂离心，使管壁上的溶液收集到管底。

试剂	10 μl反应体系	终浓度
RNA Template	X μl	1 pg～0.5 µg ¹⁾
5×EasyQuick RT MasterMix ²⁾	2 μl	1×
RNase-Free Water	up to 10 μl	/

注意：

1）如果总RNA量大于1 µg，请按比例扩大反应体系。

2）5×EasyQuick RT MasterMix 中含有Oligo(dT)、Random primer、RNase Inhibitor、dNTP Mixture、EQ-RT Buffer等。

3．37℃孵育15分钟。

4．85℃孵育5秒钟，使逆转录酶失活。

5．短暂离心后置于冰上，再进行后续实验，如果需要长时间保存，请置于-20℃。

This product is a mixture of fast reverse transcription reagents. The 5 x EasyQuick RT MasterMix contains all the reagents required for reverse transcription from RNA templates to cDNA first strand, including EasyQuick RT Reversase, RNase Inhibitor, Random 6 mers, Oligo dT Primer, dNTP, EQ-RT Buffer, etc. The reverse transcription efficiency of this product is high, and it can perform a good reverse transcription reaction on a small amount of RNA templates. The fluorescence quantitative template cDNA first strand synthesis can be completed in 15 minutes. This reagent kit is very convenient and fast to operate, and only RNA templates and water need to be added for reverse transcription reaction, making it particularly suitable for high-throughput detection.

E665905	Component	200 T	Storage
E665905A	5×EasyQuick RT MasterMix	400 μL	-20℃. Avoid freeze/thaw cycle.
E665905B	RNase-Free Water	2×1 mL	-20℃. Avoid freeze/thaw cycle.

Product features
1. Convenience: The ready to use reverse transcription Mix only requires the addition of RNA templates and water to initiate the reaction.
2. Fast: Complete cDNA first strand synthesis in 15 minutes.
3. High reverse transcription efficiency: The reverse transcription efficiency is above 90%.
4. High sensitivity: PG level templates can also obtain high-quality cDNA.
5. Read through complex templates: templates with high GC content and complex secondary structures.

Matters needing attention
1. During the operation, RNase contamination should be avoided to prevent RNA degradation or cross contamination during experiments. It is recommended that operators wear masks and disposable gloves, frequently change gloves, and use specialized instruments and consumables.
2. The reverse transcription system is prepared on ice for operation to prevent RNA degradation. The MasterMix of the reagent kit should be stored at -20 ℃ as soon as possible after use, and repeated freeze-thaw should be avoided as much as possible.
3.10 μ The reaction system can be used up to 1 μ G Total RNA, if the amount of template RNA is greater than 1 μ g. Please expand the reaction system proportionally.
4. For RNA templates with complex secondary structures, it is recommended to incubate the template RNA at 65 ℃ for 5 minutes on ice before proceeding with the next step, followed by brief centrifugation.

Operation steps
1. Thaw the template RNA on ice; After thawing the components of the reagent kit at room temperature, immediately place them on ice. Before use, vortex shake and mix each solution, and centrifuge briefly before use.
2. Prepare the reaction system according to the following table (please prepare the reaction solution on ice), vortex shake and mix well, briefly centrifuge, and collect the solution on the tube wall to the bottom of the tube.

Reagent	10 μl Reaction system	Final concentration
RNA Template	X μl	1 pg～0.5 µg ¹⁾
5×EasyQuick RT MasterMix ²⁾	2 μl	1×
RNase-Free Water	up to 10 μl	/

Attention:
1) If the total RNA content is greater than 1 µ g, please expand the reaction system proportionally.
2) 5 x EasyQuick RT MasterMix contains Oligo (dT), Random Prime, RNase Inhibitor, dNTP Mixture, EQ-RT Buffer, etc.
3. Incubate at 37 ℃ for 15 minutes.
4. Incubate at 85 ℃ for 5 seconds to inactivate reverse transcriptase.
5. After a brief centrifugation, place it on ice for subsequent experiments. If it needs to be stored for a long time, please place it at -20 ℃.

规格

别名

EasyQuick RT 主混合物

英文名称

EasyQuick RT MasterMix

储存条件

-20°C储存,避免反复冻融

运输条件

超低温运输

名称和识别符

分子类型

小分子

技术文档

📋 安全数据表 (SDS)

全面的危险、操作、储存及法规合规文件。

下载 SDS →

✅ 分析证书 (COA)

批次质量数据。输入批号以获取对应 COA。

查询 COA →

📊 产品数据表

产品规格和应用的快速参考摘要。

查看数据表 →

🔬 规格说明书

该级别的完整质量属性和验收标准。

查看规格说明 →

高级数据

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

技术文档和文章

e665905_easyquick rt mastermix.pdf

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

浓度

体积

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

大包装与定制包装 需要更大包装或定制规格？请联系我们的团队获取专属报价。