Name: 高灵敏度化学发光检测试剂盒
SKU: E665966
Availability: InStock

概述规格储存与运输技术文档高级数据质检证书（CoA,COO,BSE/TSE 和分析图谱) 技术文档和文章溶液计算器

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为什么选择此级别

，适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

2-8°C储存,避光。低温运输。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

概述

产品内容

E665966	Component	50 mL	250 mL	Storage
E665966A	eECL-A (Luminol Enhancer)	25 mL	125 mL	2-8℃.Protect from light.
E665966B	eECL-B (Peroxide)	25 mL	125 mL	2-8℃.Protect from light.

产品简介

高灵敏度化学发光检测试剂盒是免疫印迹实验中与辣根过氧化物酶（HRP）配套使用的的高灵敏增强型检测试剂盒。该产品基于新一代增强型化学发光底物研制而成，在HRP的催化下发生化学反应而发光，可用于检测固定在膜上的蛋白质等生物大分子。其高灵敏度能够检测pg级别的抗原，发光信号强烈持久，可以使用X-光胶片曝光或者化学发光成像仪进行检测。

注意事项

1. 在与膜发生接触过程中，请佩戴手套且使用干净镊子等洁净器材，避免蛋白污染及高背景。

2. 避光条件下，配制好的化学发光检测底物工作液可在室温下稳定保存8小时。阳光或其他强光会影响工作液，故应避免强光长时间的照射。正常实验室灯光短时间照射不影响工作液的使用。

3.阿拉丁试剂提供多种蛋白转移用膜、封闭液、一抗、酶标二抗、缓冲液等，详情请查询公司网站。

操作步骤

1. 二抗孵育完毕后，将印迹膜充分洗涤。

2. 根据需要量，将eECL-A和eECL-B按照1：1的比例等体积混合，配制为化学发光检测底物工作液（一张8cm×6cm的膜大约使用1ml工作液）。

3. 弃去洗涤缓冲液，将发光底物工作液滴加在印迹膜上，确保工作液覆盖整张膜，室温孵育3-5分钟。

4. 用移液器吸去多余发光底物工作液，将印迹膜置于两层干净的塑料薄膜之间，此过程应小心完成，避免膜与膜之间产生气泡。

5. 在暗室内进行X-光胶片曝光，或将膜放置到化学发光成像仪内，按照仪器说明书进行检测。

附表

Product content

E665966	Component	50 mL	250 mL	Storage
E665966A	eECL-A (Luminol Enhancer)	25 mL	125 mL	2-8℃.Protect from light.
E665966B	eECL-B (Peroxide)	25 mL	125 mL	2-8℃.Protect from light.

Products

The High Sensitivity Chemiluminescent Assay is a highly sensitive and enhanced assay for use with horseradish peroxidase (HRP) in immunoblotting experiments. It is based on a new generation of enhanced chemiluminescent substrate, which is catalyzed by HRP to emit light, and can be used to detect biological macromolecules such as proteins immobilized on membranes. The high sensitivity enables the detection of pg-level antigens with a strong and long-lasting luminescence signal, which can be detected by X-ray film exposure or chemiluminescence imaging.

Caveat

1. Please wear gloves and use clean equipment such as tweezers during the contact with the membrane to avoid protein contamination and high background.

2. Under the condition of avoiding light, the prepared working solution of chemiluminescence detection substrate can be stored stably at room temperature for 8 hours. Sunlight or other strong light may affect the working solution, so avoid prolonged exposure to strong light. Normal laboratory lighting for a short period of time does not affect the use of the working solution.

3. Aladdin Reagent offers a wide range of membranes for protein transfer, containment solutions, primary antibodies, enzyme-labeled secondary antibodies, buffers, etc. For details, please check the company's website.

Procedure

1. After incubation of the secondary antibody, the blotting membrane is washed thoroughly.

2. According to the required amount, eECL-A and eECL-B are mixed in equal volumes at a ratio of 1:1, and prepared as a chemiluminescence detection substrate working solution (about 1m working solution is used for an 8cm×6cm membrane).

3. Discard the washing buffer, add the luminescent substrate working solution dropwise onto the blotting membrane, make sure the working solution covers the whole membrane, and incubate for 3-5 minutes at room temperature.

4. Pipette off excess luminescent substrate working solution and place the blotting membrane between two layers of clean plastic film, this process should be completed carefully to avoid air bubbles between the membrane and the membrane.

5. X-ray film exposure is performed in a dark room, or the membrane is placed inside a chemiluminescence imager and tested according to the instrument instructions.

Schedules

concern	Possible causes	cure
Film reversal (white stripes, black background)	) Excess HRP in the system	Dilute the HRP marker at least 10-fold.
Brown or yellow bands appear on the membrane
Strong glow seen in dark room
Short duration of light signal
Weak or no signal	Excessive HRP in the luminescent reaction system, eliminating Excessive substrate depletion, causing rapid signal reduction	Dilute the HRP marker at least 10-fold.
	Insufficient amount of antigen/antibody	Increased antigen/antibody usage
	Low protein transfer rate	Optimization of the transfer system
high context	Excess HRP in the system	Dilute the HRP marker at least 10-fold.
	insufficiently closed	Optimization of closure procedures
	Poor choice of containment reagents	Select another containment reagent
	Inadequate rinsing	Increase rinsing time, frequency
	overexposure	Reduced exposure time
	High antigen/antibody concentration	Reduce the concentration of antigen/antibody used
Protein bands are punctate	Protein transfer failure	Optimizing the transfer process
	Membrane not in equilibrium	Handle the membrane according to the instructions
	Air bubbles between film and membrane	Remove all air bubbles before exposure
Presence of non-specific bands (high background, short signal duration)	Too much HRP in the system	Dilution of HRP markers
Presence of non-specific bands (background clean signal maintenance time normal)	Excessive use of monoclonal antibody	Further dilution of primary antibody
	SDS leads to non-specific binding	Avoid using SDS during experiments

规格

英文名称

eECL Western Blot Kit

储存与运输

储存条件

2-8°C储存,避光

运输条件

低温运输

技术文档

📋 安全数据表 (SDS)

全面的危险、操作、储存及法规合规文件。

下载 SDS →

✅ 分析证书 (COA)

批次质量数据。输入批号以获取对应 COA。

查询 COA →

📊 产品数据表

产品规格和应用的快速参考摘要。

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🔬 规格说明书

该级别的完整质量属性和验收标准。

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高级数据

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到3个结果

批号(Lot Number)	证书类型	货号
ZJ25F0521893	分析证书	E665966
ZJ25F0420894	分析证书	E665966
ZJ24F1114453	分析证书	E665966

技术文档和文章

e665966_eecl western blot kit product manual.pdf

e665966_高灵敏度化学发光检测试剂盒产品说明书.pdf

Western Blot

目录：技术文章

探索主题:

蛋白质印迹

Western Blot

目录：技术文章

探索主题:

Western blot

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

浓度

体积

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

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