凝胶迁移实验又称凝胶阻滞实验或电泳迁移率实验 (EMSA, electrophoretic mobility shift assay)是一种用于蛋白与核酸相互作用的技术。最初是用于转录因子与启动子相互作用的验证性实验，也可应用与蛋白-DNA、蛋白-RNA互作研究。
阿拉丁的UltraBio™ EMSA Precast PAGE Gel是一款安全、快捷、高性能的预制聚丙烯酰胺凝胶。
产品特点：
⁕ 采用玻璃胶板，有效减少蛋白非特异性吸附。蛋白条带更为敏锐，清晰。
⁕ 采用自动化灌胶生产技术，确保产品质量的高稳定性和重复性。
⁕ 胶夹打开极为轻松，只需用刀片在胶夹一侧轻轻划一下即可打开。
⁕ 兼容Bio-Rad、Invitrogen、天能、君意东方、北京六一等主流mini电泳槽。
基本信息：
胶板尺寸：宽×高×厚度为100×84×4.6mm
孔数：10孔，15孔
凝胶尺寸：宽×高×厚度为81×74×1.5mm
凝胶厚度：1.5mm
最大上样量：60μL，30μL
浓度：4%，6%
包装：10片/盒
预制胶选择指导：
货号
| 预制胶浓度
| 孔数
| 最大上样量
| 电泳液
| 建议电压
|
E753270-A1-10EA
| 4%
| 10
| 60 μl
| 0.5xTBE
| 100 V
|
E753270-B1-10EA
| 4%
| 15
| 30 μl
| 0.5xTBE
| 100 V
|
E753270-A2-10EA
| 6%
| 10
| 60 μl
| 0.5xTBE
| 100 V
|
E753270-B2-10EA
| 6%
| 15
| 30 μl
| 0.5xTBE
| 100 V
|
使用说明：
1. 将UltraBio™ EMSA Precast PAGE Gel 从包装袋中取出，固定在电泳槽中，安装好电泳装置。
2. 向电泳槽中加入0.5×TBE running buffer。内槽加满电泳缓冲液，外槽电泳缓冲液需至少加至1/3液面，最高不可漫过胶板，然后缓慢拔出梳子。
3. 用移液器轻轻吹打加样孔，清除残余胶液。
4. 上样：将样品与4×EMSA/Gel-Shift上样缓冲液(LB4)进行3：1混合均匀，直接上样。上样时注意枪头勿刺破凝胶或插入过深使胶板变形造成后续电泳时漏液。
5. 接通电源，恒压条件下跑胶（100V），当溴酚蓝指示带电泳至胶板底部，或实验预定位置时，即可结束电泳。
6. 电泳结束，取出凝胶。用刀在侧边胶处，沿着两片玻璃的缝隙切开封胶材料，即可打开玻璃板，取胶时，需在凝胶和玻璃条之间，沿着玻璃条划一刀，防止取胶时，发生粘连使凝胶破碎（使用美工刀时请注意安全！）。
拆胶：
1. 先沿侧边胶处简单划一刀（或先将玻璃板侧边多余封胶材料去除）；
2. 用刀在侧边胶处，沿着玻璃板和玻璃条的缝隙切开封胶材料（箭头处），轻轻打开玻璃板；
3. 取胶时，需在凝胶和两侧玻璃条之间沿着玻璃条划一刀，防止取胶时发生粘连使凝胶破碎。
UltraBio™ EMSA Precast PAGE Gel 兼容的电泳槽：
UltraBio™ EMSA Precast PAGE Gel 可以兼容大部分的 mini SDS-PAGE 电泳槽，包括
a. Bio-Rad Mini-PROTEAN (II/3 /Tetra System)；
b. Hoefer Mighty Small (SE 250/ SE 260/ SE 280)；
c. Life Technology Novex Mini-Cell (请与阿拉丁特制挡板 01配合使用)；
d. Life Mini Gel Tank 小型胶电泳槽 (请与阿拉丁特制挡板 02配合使用)；
e. 北京六一 DYCZ-25E、DYCZ-24K、DYCZ-24KS、DYCZ-24KF；
f. 君意东方 JY-SCZ2+；
g. 天能 VE180；
或其它胶板宽度在10厘米的电泳槽。
在 Life 电泳槽中的应用：如有需要，请在订购本产品时告知
将挡板01置于凝胶的外侧 将挡板02置于凝胶的内侧
在 Bio-Rad 电泳槽中的应用：
Bio-Rad Mini-PROTEAN 系列电泳槽的U型密封条顶部有凸起结构，而阿拉丁 UltraBio™ 系列预制胶的短玻板是凹形结构，因此该部位是平的，电泳前需将具有凸起结构的密封条取出后反过来安装，是平滑面朝外，从而防止漏液（如下图所示）。
a. 将 Bio-Rad 电泳槽中的U型密封条（如图绿色部分）拉出，注意这时的密封条两端是有凸起的，凸起的这面为正面，无凸起的为反面。
b. 将密封条旋转180度 (正面朝里，反面朝外)，重新装回电泳槽中，注意把密封圈周边压实，防止发生漏液。
c. 放置好预制胶进行正常的电泳操作即可。
注意事项：
1. 电泳缓冲液不建议重复使用。因为经过电泳之后，缓冲液中的离子强度、pH值和缓冲能力都发生了变化，不能确保电泳效果（DNA电泳产生条带模糊和不规则的DNA带迁移的现象）。
2. 仅限于专业人员的科学研究用，不得用于临床医学诊断及其他非合理用途。不得存放于普通住宅内。
3. 为了您的安全和健康，请穿戴好个人防护装备和实验服装进行操作。
The Gel Shift Assay, also known as the Electrophoretic Mobility Shift Assay (EMSA), is a powerful technique used to study protein-nucleic acid interactions. Initially developed to verify the interaction between transcription factors and promoters, it has been widely adapted to investigate various other protein-DNA and protein-RNA interactions.
Aladdin's UltraBio™ EMSA Precast PAGE Gel is a safe, efficient, and high-performance precast polyacrylamide gel system designed specifically for EMSA experiments.
Product Features:
⁕ Made with glass plates to effectively reduce non-specific protein adsorption, resulting in sharper and clearer protein bands.
⁕ Manufactured using automated gel casting technology to ensure high stability and reproducibility of product quality.
⁕ The gel cassettes are extremely easy to open – simply make a light scratch on one side with a blade.
⁕ Compatible with mainstream mini electrophoresis tanks from Bio-Rad, Invitrogen, Tanon, Junyi East, Beijing Liuyi, and other leading manufacturers.
Basic Information:
Gel Plate Dimensions: Width × Height × Thickness = 100 × 84 × 4.6 mm
Number of Wells: 10 wells, 15 wells
Gel Dimensions: Width × Height × Thickness = 81 × 74 × 1.5 mm
Gel Thickness: 1.5 mm
Maximum Sample Loading: 60 μL, 30 μL
Concentration: 4%, 6%
Packaging: 10 pieces/box
Precast Gel Selection Guide:
E753270
| Precast Gel Concentration
| Number of Wells
| Maximum Sample Loading Volume
| Buffer
| The recommended voltage
|
E753270-A1-10EA
| 4%
| 10
| 60 μl
| 0.5xTBE
| 100 V
|
E753270-B1-10EA
| 4%
| 15
| 30 μl
| 0.5xTBE
| 100 V
|
E753270-A2-10EA
| 6%
| 10
| 60 μl
| 0.5xTBE
| 100 V
|
E753270-B2-10EA
| 6%
| 15
| 30 μl
| 0.5xTBE
| 100 V
|
Usage Instructions:
1. Remove the UltraBio™ EMSA Precast PAGE Gel from its packaging, secure it in the electrophoresis tank, and assemble the electrophoresis apparatus.
2. Add 0.5×TBE running buffer to the electrophoresis tank. Fill the inner chamber completely with electrophoresis buffer. Add buffer to the outer chamber to at least 1/3 of the liquid level, but ensure it does not overflow the top of the gel plate. Then, gently remove the comb.
3. Gently pipette up and down in the sample wells to remove any residual gel pieces.
4. Loading Samples: Mix the sample with 4×EMSA/Gel-Shift Loading Buffer (LB4) in a 3:1 ratio and load directly. When loading, ensure the pipette tip does not pierce the gel or insert too deeply, which could cause the gel plate to deform and lead to buffer leakage during subsequent electrophoresis.
5. Connect the power supply and run the gel at a constant voltage (100V). The electrophoresis can be stopped when the bromophenol blue indicator has migrated to the bottom of the gel plate or to the predetermined position in the experiment.
6. After electrophoresis, remove the gel. To open the glass plates, use a knife to cut through the sealing material along the gap between the two glass plates on the side. To remove the gel, make a cut along the glass strips between the gel and the glass plates to prevent adhesion and breakage (please use extreme caution when using a utility knife!).
Gel Disassembly:
1. First, make a simple cut along the side gel (or remove excess gel-sealing material from the sides of the glass plate first);
2. Use a knife to cut through the gel-sealing material along the gap between the glass plate and the glass strip at the side gel (at the arrow), then gently open the glass plate;
3. When removing the gel, make a cut along the glass strips between the gel and the glass strips on both sides to prevent the gel from sticking and breaking during removal.
Compatible Electrophoresis Tanks for UltraBio™ EMSA Precast PAGE Gel:
UltraBio™ EMSA Precast PAGE Gel is compatible with most mini SDS-PAGE electrophoresis tanks, including:
a. Bio-Rad Mini-PROTEAN (II/3 /Tetra System)
b. Hoefer Mighty Small (SE 250/ SE 260/ SE 280)
c. Life Technology Novex Mini-Cell (to be used with Aladdin Custom Baffle 01)
d. Life Mini Gel Tank (to be used with Aladdin Custom Baffle 02)
e. Beijing Liuyi DYCZ-25E, DYCZ-24K, DYCZ-24KS, DYCZ-24KF
f. Junyi East JY-SCZ2+
g. Tanon VE180
or other electrophoresis tanks with a gel plate width of 10 cm.
Application in Life Electrophoresis Tanks: If required, please inform us when ordering this product
Place Baffle No. 01 on the outer side of the gel Place Baffle No. 02 on the inner side of the gel
Application in Bio-Rad Electrophoresis Tanks:
The top of the U-shaped sealing strip in the Bio-Rad Mini-PROTEAN series electrophoresis tanks has a raised structure, while the short glass plate of Aladdin’s UltraBio™ series precast gels has a concave structure—resulting in a flat surface at this location. Before electrophoresis, the sealing strip with the raised structure must be removed and reinstalled in reverse, with the smooth side facing outward, to prevent liquid leakage (as shown in the figure below).
a. Pull out the U-shaped sealing strip (the green part in the figure) from the Bio-Rad electrophoresis tank. Note that the two ends of the sealing strip have protrusions at this point: the side with protrusions is the front side, and the side without protrusions is the back side.
b. Rotate the sealing strip 180 degrees (with the front side facing inward and the back side facing outward) and reinstall it in the electrophoresis tank. Ensure the perimeter of the sealing ring is pressed firmly to prevent liquid leakage.
c. Place the precast gel and proceed with normal electrophoresis operations.
Precautions:
1. Reuse of electrophoresis buffer is not recommended. After electrophoresis, ionic strength, pH and buffering capacity of the buffer will change, which cannot guarantee optimal electrophoresis performance (DNA electrophoresis may result in blurred bands and irregular DNA band migration).
2. For scientific research use only by professional personnel. Not for clinical diagnosis or other improper purposes. Do not store in ordinary residential premises.
3. For your safety and health, please wear personal protective equipment and laboratory clothing during operation.
ZJ26F0332663
危险品化学品经营许可证（带存储）