计算溶液所需的质量、体积或浓度。
BioReagent,无菌,用于细胞培养 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
避光,-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
储存缓冲液:
A sterile solution containing NaCl and streptomycin sulfate.
复溶:
Add 0.5 mL of sterile PBS or ddH2O to the vial and gently swirl until the contents are dissolved, resulting in a stock solution of 30 mg/mL ECGS.
产品介绍:
内皮细胞生长补充剂 (ECGS) 是一种包含哺乳动物血管内皮细胞生长促进因子的牛神经组织提取物,能促进人脐静脉内皮细胞 (HUVEC) 的增殖。它可与白介素 (IL-1) 协调并介导血管平滑肌细胞 (VSMC) 中的基质重塑。ECGS作为一种培养基补充剂,可对用于单克隆抗体生产的杂交瘤细胞的融合和生长起到积极作用。
使用说明:
内皮细胞生长补充剂 (ECGS) 是通过对Maciag等人的方法今夕修改而制备的,由含有NaCl和硫酸链霉素的无菌溶液冻干而成。ECGS以无菌冻干粉形式提供,每瓶含15mg蛋白质。
使用时,向小瓶中加入0.5 mL无菌PBS或ddH2O,轻轻搅拌直至溶解,即可得到30 mg/mL ECGS的储备溶液。该储备溶液应在无菌组织培养基中进一步稀释,以达到所需的工作浓度。促进血管内皮细胞生长的最佳浓度范围为10-300 μg/mL。
注意事项:
1、收到产品后,将其重新配制,然后分装成小体积,在﹣20℃下保存6个月,或在 2-8℃ 下保存不超过一周。避免反复冻融。
2、本产品仅供专业科研人员使用,不得用于临床诊断或治疗、食品或其他用途。
3、为了您的安全和健康,请在操作过程中穿戴白大褂和一次性手套。
Endothelial Cell Growth Supplement (ECGS) is a bovine neural tissue extract containing growth factors for mammalian vascular endothelial cells, which can promote the proliferation of Human Umbilical Vein Endothelial Cells (HUVECs). In combination with interleukin (IL-1), ECGS mediates matrix remodeling in Vascular Smooth Muscle Cells (VSMCs). As a cell culture supplement, it also exerts positive effects on the fusion and growth of hybridoma cells for monoclonal antibody production.
Instructions for Use:
ECGS is prepared by modifying the method established by Maciag et al. It is supplied as a lyophilized powder from a sterile solution containing sodium chloride and streptomycin sulfate. Each vial contains 15 mg of total protein. For reconstitution: Add 0.5 mL of sterile PBS or double-distilled water to the vial and mix gently until fully dissolved to prepare a stock solution at 30 mg/mL. Further dilute the stock solution in sterile tissue culture medium to the desired working concentration. The optimal concentration for vascular endothelial cell growth ranges from 10 to 300 μg/mL.
Precautions:
1. After receiving the product, reconstitute it, aliquot into small portions, and store at -20 °C for up to 6 months, or at 2–8 °C for no more than one week. Avoid repeated freeze-thaw cycles.
2. This product is intended for research use only by professional researchers. It shall not be used for clinical diagnosis, treatment, food production or other purposes.
3. Wear a lab coat and disposable gloves throughout the operation for personal safety and health protection.
通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | E767638 | |
| 分析证书 | E767638 | |
| 分析证书 | E767638 | |
| 分析证书 | E767638 | |
| 分析证书 | E767638 |
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