计算溶液所需的质量、体积或浓度。
外泌体定量检测试剂盒 (ELISA 夹心法)
有货
为什么选择此级别
BioReagent,无菌 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存,避光,-20°C储存。低温运输,超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
本产品是基于 ELISA夹心法用于外泌体定性定量的检测试剂盒，利用高性能 CD9捕获抗体和 CD81检测抗体可以灵敏地检测共表达外泌体表面标记的 CD9和 CD81靶标。本产品可适用于人 体体液、细胞培养液等多种样本，能够实现对原始样本中的外泌体进行快速精准定量。
| E778166 | 组分 | 24T | 48T | 96T | 储存 |
| E778166A | 捕获抗体包被板 | 24T | 48T | 96T | 2-8°C |
| E778166B | Exosomes标准品S1 | 1 mL | 1 mL | 1 mL | -20°C |
| E778166C | 酶标记物 | 2.5 mL | 5 mL | 10 mL | 2-8°C |
| E778166D | 显色液A | 1.5 mL | 3 mL | 6 mL | 2-8°C. 避光 |
| E778166E | 显色液B | 1.5 mL | 3 mL | 6 mL | 2-8°C. 避光 |
| E778166F | 终止液 | 1.5 mL | 3 mL | 6 mL | 2-8°C |
| E778166G | 浓缩洗液(20X) | 7.5 mL | 15 mL | 30 mL | 2-8°C |
| E778166H | 稀释液 | 1.25 mL | 2.5 mL | 5 mL | 2-8°C |
| E778166I | Exosomes标准品S2 | 1 mL | 1 mL | 1 mL | -20°C |
| E778166J | Exosomes标准品S3 | 1 mL | 1 mL | 1 mL | -20°C |
| E778166K | Exosomes标准品S4 | 1 mL | 1 mL | 1 mL | -20°C |
| E778166L | Exosomes标准品S5 | 1 mL | 1 mL | 1 mL | -20°C |
产品特点：
应用广泛：适用于细胞培养上清、尿液、血清等多种类型样本；
操作简单：仅需1台450 nm 酶标仪，无需其它任何设备；
稳定性、重现性高：为了实现稳定性和再现性，试剂盒使用了CD9/CD81融合蛋白标准品，而非不稳定、难以储存的外泌体本身。
外泌体完整性检测：可用于外泌体完整性快速评估。
产品原理：
图1. 基于ELISA的外泌体定量检测原理示意图
检验流程：
1、检测前准备工作
①所有试剂应在使用前室温平衡至少15min。
②取浓缩洗液 (20X)，用蒸馏水或去离子水将20X浓缩洗液稀释成1X工作液，混匀备用。(试剂盒提供稀释液，可自行用高浓度标准品稀释使用)
2、操作步骤
①准备已平衡好的板条和试剂，试剂充分摇匀。
②微孔板中分别加入各浓度Exosomes标准品以及待测样本 (100μL/孔)。
③用封板膜封住反应孔，于37°C恒温条件下，避光孵育60min。
④孵育结束后，加入工作液洗液清洗3次，每次200-250μL/孔，洗板结束后在吸水纸上拍干。
⑤每孔加入100μL酶标记物，用封板膜封住反应孔，于37°C恒温条件下，避光孵育60min。
⑥孵育结束后，加入工作液洗液清洗3次，每次200-250μL/孔，洗板结束后在吸水纸上拍干。
⑦配制显色底物工作液：将显色液A和B等比例混合得显色底物工作液 (请配制完2min内使用)。
⑧各反应孔加入100μL显色底物液工作液，37°C恒温条件下避光反应30min。
⑨各反应孔加入50μL终止液，混匀后即刻测量OD₄₅₀值 (3min内)，并绘制XY线性曲线。
3、校准程序
采用XY线性拟合，确保线性相关系数R²≥0.99。每次进行实验时，必须进行完整的校准品操作，以绘制校准曲线。
| 校准点 | S1 | S2 | S3 | S4 | S5 |
| 浓度(ng/mL) | 0 | 7.816 | 15.625 | 31.25 | 45 |
| OD₄₅₀ | 0.0737 | 0.3074 | 0.5183 | 0.8618 | 1.2945 |
注意：本图仅供参考，应以同次试验标准品所绘标准曲线计算样本中 Exosome的含量
注意事项和免责说明
1、本试剂盒需避光保存。
2、本品仅限于专业人员的科学研究使用，不得用于临床诊断或治疗，不得用于食品或药品。
This product is an exosome qualitative and quantitative detection kit based on the sandwich ELISA method. It adopts high-performance CD9 capture antibody and CD81 detection antibody to sensitively detect CD9 and CD81 targets co-expressed on the surface of exosomes. Compatible with various samples such as human body fluids and cell culture media, this kit enables rapid and accurate quantification of exosomes in raw samples.
| E778166 | Component | 24T | 48T | 96T | Storage |
| E778166A | Capture Antibody-Coated Microplate | 24 wells | 48 wells | 96 wells | 2-8°C |
| E778166B | Exosome Standard S1 | 1 mL | 1 mL | 1 mL | -20°C |
| E778166C | Enzyme Conjugate | 2.5 mL | 5 mL | 10 mL | 2-8°C |
| E778166D | Chromogenic Solution A | 1.5 mL | 3 mL | 6 mL | 2-8°C. Store in the dark. |
| E778166E | Chromogenic Solution B | 1.5 mL | 3 mL | 6 mL | 2-8°C. Store in the dark. |
| E778166F | Stop Solution | 1.5 mL | 3 mL | 6 mL | 2-8°C |
| E778166G | Concentrated Wash Buffer (20×) | 7.5 mL | 15 mL | 30 mL | 2-8°C |
| E778166H | Assay Diluent | 1.25 mL | 2.5 mL | 5 mL | 2-8°C |
| E778166I | Exosome Standard S2 | 1 mL | 1 mL | 1 mL | -20°C |
| E778166J | Exosome Standard S3 | 1 mL | 1 mL | 1 mL | -20°C |
| E778166K | Exosome Standard S4 | 1 mL | 1 mL | 1 mL | -20°C |
| E778166L | Exosome Standard S5 | 1 mL | 1 mL | 1 mL | -20°C |
Product Features:
Wide Application: Compatible with various sample types including cell culture supernatant, urine, serum, etc.
Easy Operation: Only a microplate reader with 450 nm wavelength is required; no additional equipment is needed.
Excellent Stability and Reproducibility: This kit adopts CD9/CD81 fusion protein standards instead of native exosomes, which are unstable and hard to preserve, to guarantee superior stability and repeatability.
Exosome Integrity Assay: Enables rapid evaluation of exosome integrity.
Product Principle:
Test Procedure:
1. Pre-test Preparation
① All reagents shall be equilibrated to room temperature for at least 15 minutes before use.
② Dilute the 20× Concentrated Wash Buffer with distilled or deionized water to prepare 1× working solution, mix thoroughly for later use. (The kit is supplied with assay diluent, which can be used to dilute high-concentration standards as required.)
2. Operating Steps
① Take the equilibrated microplate strips and reagents, and fully shake all reagents evenly.
② Add serially diluted Exosome Standards and test samples into respective wells of the microplate (100 μL per well).
③ Seal the reaction wells with plate sealer, and incubate at a constant temperature of 37 °C away from light for 60 min.
④ After incubation, wash wells with wash working solution 3 times, 200–250 μL per well each time. Blot dry on absorbent filter paper after washing.
⑤ Add 100 μL Enzyme Conjugate to each well, seal the plate, and incubate at 37 °C in the dark for 60 min.
⑥ Repeat the washing procedure 3 times as described above, then blot dry completely.
⑦ Prepare chromogenic substrate working solution: mix Chromogenic Solution A and Solution B at an equal volume ratio. Use the mixed substrate within 2 minutes after preparation.
⑧ Pipette 100 μL freshly prepared chromogenic substrate working solution into each well, and incubate at 37 °C protected from light for 30 min.
⑨ Add 50 μL Stop Solution to each well, mix gently, and measure OD₄₅₀ values immediately (within 3 min). Plot an XY linear calibration curve.
3. Calibration Protocol
XY linear fitting is adopted, with a mandatory requirement that the coefficient of determination R² ≥ 0.99. A full set of calibrants must be tested in each independent experiment to generate the calibration curve.
| 校准点 | S1 | S2 | S3 | S4 | S5 |
| Concentration (ng/mL) | 0 | 7.816 | 15.625 | 31.25 | 45 |
| OD₄₅₀ | 0.0737 | 0.3074 | 0.5183 | 0.8618 | 1.2945 |
Exosome Standard Calibration Curve
Concentration (ng/mL)
Note: This figure is for reference only. The concentration of exosomes in samples shall be calculated based on the standard curve generated from standards tested in the same assay run.
Precautions and Disclaimer
1. This kit shall be stored away from light.
2. This product is intended solely for scientific research use by professionals. It must not be used for clinical diagnosis, clinical treatment, food or pharmaceutical applications.
规格
别名
外泌ELISA试剂盒
英文别名
Exosome ELISA Kit
规格或纯度
BioReagent, 无菌
英文名称
Exosome Assay Kit (ELISA Sandwich Method)
敏感性
Light-sensitive
储存与运输
储存条件
2-8°C储存,避光,-20°C储存
运输条件
低温运输,超低温运输
稳定性与储存
各组分在相应的储存条件下保质期12个月。
组分与储存
| 储运单元货号 | 储运单元名称 | 组分货号 | 组分名称 | 物理外观 | 24T | 48T | 96T | 储存 |
| E778166WT | 外泌体定量检测试剂盒 (ELISA 夹心法) (2-8℃组分) | E778166A | 捕获抗体包被板 | / | 24T | 48T | 96T | 2-8°C. 避光 |
| E778166C | 酶标记物 | 液体 | 2.5 mL | 5 mL | 10 mL | 2-8℃. | ||
| E778166D | 显色液A | 液体 | 1.5 mL | 3 mL | 6 mL | 2-8°C. 避光 | ||
| E778166E | 显色液B | 液体 | 1.5 mL | 3 mL | 6 mL | 2-8°C. 避光 | ||
| E778166F | 终止液 | 液体 | 1.5 mL | 3 mL | 6 mL | 2-8℃. | ||
| E778166G | 浓缩洗液 (20×) | 液体 | 7.5 mL | 15 mL | 30 mL | 2-8℃. | ||
| E778166H | 稀释液 | 液体 | 1.25 mL | 2.5 mL | 5 mL | 2-8℃. | ||
| E778166DT | 外泌体定量检测试剂盒 (ELISA 夹心法) (-20℃组分) | E778166B | Exosomes标准品S1 | 液体 | 1 mL | 1 mL | 1 mL | -20℃. |
| E778166I | Exosomes标准品S2 | 液体 | 1 mL | 1 mL | 1 mL | -20℃. | ||
| E778166J | Exosomes标准品S3 | 液体 | 1 mL | 1 mL | 1 mL | -20℃. | ||
| E778166K | Exosomes标准品S4 | 液体 | 1 mL | 1 mL | 1 mL | -20℃. | ||
| E778166L | Exosomes标准品S5 | 液体 | 1 mL | 1 mL | 1 mL | -20℃. |
注：发货时各组分依据储存温度（2‑8℃/-20℃）独立包装，差异化温控运输以保障产品性能。同一订单含多温区物料可能分批次送达，请以实际收货为准。收货后按组分表标注温度分类存放，有效期自生产之日起 12 个月。
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 G2624345 |
分析证书 | E778166 |
| G2613370 |
分析证书 | E778166 |
| ZJ25F0927272 |
分析证书 | E778166 |
| ZJ25F0927271 |
分析证书 | E778166 |
| F2626288 |
分析证书 | E778166 |
技术文档和文章
溶液计算器
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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