计算溶液所需的质量、体积或浓度。
BioReagent,无菌 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
避光,-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
本产品是阿拉丁经过优化配方,重磅推出的针对外泌体样本研发的裂解试剂,可高效裂解外泌体蛋白。具有操作简便且裂解效率高等优点,使用时与样本 1:1添加混匀即可,裂解后的蛋白样品可用于 BCA、Western Blot和蛋白组学检测等实验。
操作流程:
1.实验准备:实验前先将本产品从 -20℃冷冻环境中取出后进行化冻,将裂解液融化混匀后置于冰上。
2.将外泌体样本与裂解液按体积比 1:1添加混匀,95℃煮 10min。
3.离心(3000g、5min),取上清,进行BCA蛋白浓度测定、Western Blot和蛋白组学检测等实验。
注意事项和免责说明:
1.本产品不含有蛋白酶抑制剂,根据实验需要自行添加,避免反复冻融,建议分装存储。
2.为保证裂解充分,裂解后蛋白浓度应小于 2μg/μL。
3.本试剂要避光保存。
4.为了您的安全和健康,请穿实验服并戴一次性手套操作。
5.本产品仅限专业人员的科学研究使用,不得用于临床诊断或治疗,不得用于食品或药品。
Product Introduction:
This product is a lysate reagent developed by Aladdin with an optimized formula, specifically targeting exosome samples, which can efficiently lyse exosomal proteins. It features advantages such as simple operation and high lysis efficiency. When in use, it can be added to the sample at a 1:1 ratio and mixed well. The lysed protein samples can be used in experiments such as BCA, Western Blot, and proteomics detection.
Operating Procedures:
1.Experimental preparation: Before the experiment, take this product out of the -20℃ freezing environment to thaw. After melting and mixing the lysate thoroughly, place it on ice.
2.Add the exosome sample and the lysate at a volume ratio of 1:1, mix well, and boil at 95℃ for 10 minutes.
3.Centrifuge (3000g, 5 minutes), take the supernatant, and perform experiments such as BCA protein concentration determination, Western Blot, and proteomics detection.
Notes and Disclaimer:
1.This product does not contain protease inhibitors. Please add them by yourself according to experimental needs. Avoid freeze/thaw cycle, and it is recommended to aliquot for storage.
2.To ensure sufficient lysis, the protein concentration after lysis should be less than 2μg/μL.
3.This reagent should be stored away from light.
4.For your safety and health, please wear a lab coat and disposable gloves during operation.
5.This product is limited to scientific research use by professional personnel, and shall not be used for clinical diagnosis or treatment, nor for food or pharmaceutical purposes.
通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | E778170 |