外泌体专用无血清培养基是一款用于多种肿瘤细胞、干细胞等细胞中外泌体表达的培养基。在细胞培养中不需要添加血清，但在某些应用中可能要添加生长因子或细胞因子。无血清培养基中添加了血清的主要成分：粘附因子、生长因子、必需的营养物质和激素等，能减少血清带来的不利因素，使细胞培养的条件更稳定。该培养基为无动物源、无血清配方，细胞培养过程中无需添加血清即可达到完全培养基的培养状态，有利于外泌体的收获，适用于多种细胞系和 T细胞的外泌体收集。
产品组分及储存条件：
| 储运单元货号 | 储运单元名称 | 组分货号 | 组分名称 | 物理外观 | 100mL | 500mL | 储存温度 |
| E778171WT | 外泌体专用无血清培养基 (含酚红)(2-8℃组分) | E778171A | 外泌体专用无血清培养基 | 液体 | 100mL | 500mL | 2-8℃. |
| E778171DT | 外泌体专用无血清培养基 (含酚红)(-20℃组分) | E778171B | 外泌体专用无血清培养基添加剂 | 液体 | 5mL | 25mL | -20℃.避免反复冻融 |
注：发货时各组分依据储存温度（2-8℃/-20℃）独立包装，差异化温控运输以保障产品性能。同一订单含多温区物料可能分批次送达，请以实际收货为准。收货后按组分表标注温度分类存放，有效期自生产之日起 12 个月。
产品特点：
效果稳定：高外泌体分泌量以及高活性外泌体。
适用广泛：适用于多种细胞系的培养和外泌体收集。
操作流程：
1.外泌体专用无血清培养基与添加剂采用 20:1比例混合，制成完全培养基；
2.使用完全培养基培养细胞，持续培养 24-48h，细胞汇合度达到 80-90% 时，收集上清液提取外泌体。
3.备注：完全培养基现配现用，2-8℃存储，一周内用完；添加剂如需多次配制，建议分装冻存。
注意事项和免责说明：
1.本产品适用于外泌体培养收集，可以作为细胞传代培养基使用。添加剂添加之后培养基中会出现絮状物质属正常现象，不属于污染，不影响细胞生长和外泌体提取。
2.为了您的安全和健康，请穿实验服并戴一次性手套操作。
3.本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品。
The serum-free medium specially for exosomes is a medium used for exosome expression in various cells such as tumor cells and stem cells. No serum needs to be added during cell culture, but growth factors or cytokines may be added in some applications. The serum-free medium contains the main components of serum: adhesion factors, growth factors, essential nutrients, hormones, etc., which can reduce the adverse factors caused by serum and make the cell culture conditions more stable. This medium has an animal-origin-free and serum-free formula. During cell culture, it can achieve the culture state of a complete medium without adding serum, which is beneficial to the harvest of exosomes and suitable for exosome collection from various cell lines and T cells.
Product Components and Storage Conditions:
| Storage and Transport Unit Item No. | Storage and Transport Unit Name | Component No. | Component Name | Appearance | 100mL | 500mL | Storage |
| E778171WT | Exosome-specific Serum-free Medium (with phenol red)(2-8℃ Components) | E778171A | Exosome-specific Serum-free Medium | Liquid | 100mL | 500mL | 2-8℃. |
| E778171DT | Exosome-specific Serum-free Medium (with phenol red)(-20℃ Components) | E778171B | Exosome-Specific Serum-free Medium Supplement | Liquid | 5mL | 25mL | -20℃. Avoid freeze-thaw cycle. |
Note: Each component is individually packaged according to its storage temperature (2-8℃/-20 °C) upon shipment, with differentiated temperature‑controlled transportation to guarantee product performance. Orders containing materials requiring multiple temperature zones may be delivered in separate batches; please refer to the actual received goods. Upon receipt, store components separately at the temperatures specified in the component list. The shelf‑life is 12 months from the date of manufacture.
Product Features:
Stable Effect: High exosome secretion and high-activity exosomes.
Wide Applicability: Suitable for the culture of various cell lines and exosome collection.
Operating Procedures:
1.Mix the serum-free medium specially for exosomes with the additive at a ratio of 20:1 to prepare the complete medium;
2.Culture cells with the complete medium for 24-48 hours continuously. When the cell confluency reaches 80-90%, collect the supernatant to extract exosomes.
3.Notes: The complete medium should be prepared and used immediately. Store it at 2-8°C and use it up within one week; if the additive needs to be prepared multiple times, it is recommended to aliquot and store it frozen.
Notes and Disclaimer:
1.This product is suitable for exosome culture and collection, and can be used as a cell passage medium. The appearance of flocculent substances in the medium after adding the additive is a normal phenomenon, not a contamination, and will not affect cell growth or exosome extraction.
2.For your safety and health, please wear a lab coat and disposable gloves during operation.
3.This product is intended solely for scientific research by professionals and shall not be used for clinical diagnosis or treatment, nor for food or pharmaceutical purposes.
ZJ26F0939536
危险品化学品经营许可证（带存储）