计算溶液所需的质量、体积或浓度。
外泌体保存液（2×）
有货
为什么选择此级别
BioReagent,无菌 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存,避光。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
目前，市面上的外泌体保存液存在着种类少、效果不佳等问题。它容易造成外泌体在保存过程中结构被破坏，生物活性丧失或内含物降解，影响对外泌体的后续检测、标记等下游的研究或应用。为了解决这一问题，阿拉丁研发了一款成分简单且不影响外泌体活性的保存液。它能够在低温下长期保存外泌体，适用于液态及冻干形态的保存，能够有效降低冻干对外泌体的负面影响。
产品特点
保存周期久：能够在4 ℃条件下保存至少6个月。
简单稳定：保存液成分简单，不影响外泌体活性。
适用范围广：液态和冻干均可保存。
产品数据：
1. 外泌体不同保存条件下的CD9蛋白含量
对不同保存条件下的外泌体进行ELISA蛋白定量实验，结果显示（图1），相比25℃，外泌体在4℃保存一个月的蛋白含量损失较少，且在保存液中的损失率明显降低（图2）。
2. 外泌体粒子损失率
对不同保存条件下的外泌体进行NTA检测，结果显示（图3），在室温和37℃条件下保存一个月，外泌体粒子的损失率高达80%以上。在4℃、-20℃和-80℃条件下保存一个月的外泌体粒子损失率明显低于室温和37℃。
3. 不同保存条件下外泌体的形态
与新鲜分离的外泌体相比，在PBS中4℃保存14天后的外泌体膜结构不完整，会导致外泌体蛋白的泄露以及松散结合的“外泌体外周”蛋白的解离。将外泌体保存于保存液中冻干后于-20℃保存3个月，电镜结果显示外泌体膜结构完整，形态完整清晰。
操作流程：
1.取 20μL EV Save Buffer（2×），加入 20μL ddH₂O将其稀释至 1×。
2.以 10¹¹个外泌体为例，加入 40μL EV Save Buffer（1×）后混匀保存。
注意事项和免责声明：
1.若需要短时间内取用外泌体，请将外泌体置于 4℃保存，避免冻融对外泌体的影响。
2.外泌体加入本保存液后可在 4℃存放 6个月，冻干后在 -80℃可稳定保存。
3.本产品仅限于专业人员的科学研究使用，不得用于临床诊断或治疗，不得用于食品或药品。
Product Introduction:
Currently, exosome preservation solutions available on the market have problems such as few types and poor effectiveness. They are likely to cause damage to the structure of exosomes during preservation, loss of biological activity or degradation of contents, which affects subsequent downstream research or applications such as exosome detection and labeling. To solve this problem, Aladdin has developed a preservation solution with simple components that does not affect the activity of exosomes. It can preserve exosomes for a long time at low temperatures, is suitable for the preservation of liquid and lyophilized forms, and can effectively reduce the negative impact of lyophilization on exosomes.
Product Features:
Long preservation period: Can be stored at 4°C for at least 6 months.
Simple and stable: The preservation solution has simple components and does not affect the activity of exosomes.
Wide application range: Suitable for preserving both liquid and lyophilized forms.
Product Data:
1.CD9 protein content of exosomes under different preservation conditions
ELISA protein quantification experiments were performed on exosomes under different preservation conditions. The results showed that compared with 25°C, the exosomes preserved at 4°C for one month had less protein content loss, and the loss rate in the preservation solution was significantly reduced .
2.Exosome particle loss rate
NTA detection was performed on exosomes under different preservation conditions. The results showed that when stored at room temperature and 37°C for one month, the loss rate of exosome particles was as high as more than 80%. The loss rate of exosome particles stored at 4°C, -20°C and -80°C for one month was significantly lower than that at room temperature and 37°C.
3.Morphology of exosomes under different preservation conditions
Compared with freshly isolated exosomes, the membrane structure of exosomes stored in PBS at 4°C for 14 days is incomplete, which will lead to the leakage of exosomal proteins and the dissociation of loosely bound "exosomal peripheral" proteins. When exosomes are stored in the preservation solution, lyophilized and then kept at -20°C for 3 months, electron microscopy results show that the exosomal membrane structure is intact, and the morphology is complete and clear.
Operating Procedures:
1.Take 20μL of EV Save Buffer (2×) and add 20μL of ddH₂O to dilute it to 1×.
2.Take 10¹¹ exosomes as an example, add 40μL of EV Save Buffer (1×), mix well and store.
Notes and Disclaimer:
1.If exosomes need to be used within a short period of time, store them at 4°C to avoid the impact of freezing and thawing on exosomes.
2.After adding exosomes to this preservation solution, they can be stored at 4°C for 6 months, and can be stably preserved at -80°C after lyophilization.
3.This product is limited to scientific research use by professional personnel, and shall not be used for clinical diagnosis or treatment, nor for food or pharmaceutical purposes.
规格
别名
外泌体保存液 | 外泌体储存液
英文别名
EV Save Buffe
规格或纯度
BioReagent, 无菌, 2×
稳定性与储存
2-8℃长期储存（12个月），避光。
英文名称
Exosome Preservation Solution (2×)
储存条件
2-8°C储存,避光
运输条件
低温运输
产品线
生物产品
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
化学和物理性质
敏感性
Light-sensitive
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ25F0927438 |
分析证书 | E778172 |
溶液计算器
常见问题
什么是「无菌」？
「无菌」表示该物料经过加工和检测，适用于特种工业加工。规格侧重于影响该应用性能的杂质——例如，电子应用的金属含量、电池应用的粒度、光学应用的折射率，或对水分敏感工艺的水分含量。
什么是「BioReagent」？
「BioReagent」表示该产品经过加工和检测，适用于生物和生化应用。规格可能包括内毒素限值、微生物控制、无菌性、低 DNase/RNase/蛋白酶活性，以及在相关情况下的生物活性验证。
本产品应如何储存？
请于2–8 °C、避光条件下保存。本品对光敏感，请保存在原始的避光或棕色容器中。
本产品如何运输？
本产品采用湿冰冷藏运输。收货后请立即拆包，并按上述储存条件保存。
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每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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