BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
补体片断3a(C3a)是由补体成分3裂解形成的蛋白质之一；另一个是C3b。C3a是一种有77个残基的无源性毒素，与C3a受体（C3aR）结合，是一种A类G蛋白偶联受体。它在免疫反应中起着很大的作用。C3a分子通过GPCR C3a受体诱导反应。与其他无精蛋白一样，C3a通过裂解其羧基末端的精氨酸来调节，从而产生一个具有较低炎症功能的分子（C3a去精氨酸）。它是补体系统的效应物，具有一系列功能，包括T细胞的激活和存活，刺激血管生成，趋化作用，肥大细胞脱颗粒和巨噬细胞激活。它已被证明具有促炎症和抗炎症反应，其活性能够抵消C5a的促炎症作用。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有兔补体片断3a(C3a)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的兔补体片断3a(C3a)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rabbit C3a. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rabbit C3a present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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