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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
胰蛋白酶是一种来自PA家族超家族的丝氨酸蛋白酶,存在于许多脊椎动物的消化系统中,它会将蛋白质水解为肽,进而分解为氨基酸。胰蛋白酶在小肠中形成时,其酶原形式,即胰腺产生的胰蛋白酶原被激活。胰蛋白酶主要在氨基酸赖氨酸或精氨酸的羧基侧切割肽链。胰蛋白酶由Wilhelm Kühne于1876年发现,并从古希腊语中的摩擦词命名,因为它最初是通过用甘油摩擦胰腺而分离出来的。在十二指肠中,胰蛋白酶催化肽键的水解,将蛋白质分解成更小的肽。然后肽产物通过其他蛋白酶进一步水解成氨基酸,使它们可被吸收到血流中。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有猪胰蛋白酶(Trypsin)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的猪胰蛋白酶(Trypsin)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Pig Trypsin. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Pig Trypsin present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.