BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
肿瘤坏死因子（TNF，cachexin；通常称为肿瘤坏死因子α或TNF-α）是一种脂肪因子和一种细胞因子。TNF是TNF超家族的一个成员，它由各种具有同源TNF结构域的跨膜蛋白组成。作为一种脂肪因子，TNF促进胰岛素抵抗。作为一种细胞因子，它被免疫系统用于细胞信号传递。如果巨噬细胞（某些白血球）检测到感染，它们会释放TNF以提醒其他免疫系统细胞，作为炎症反应的一部分。TNF-α以跨膜形式（mTNF-α）和可溶性形式（sTNF-α）存在。sTNF-α由mTNF-α的酶裂解产生。sTNF-α选择性地与TNFR1结合，而mTNF-α同时与TNFR1和TNFR2结合。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有猴肿瘤坏死因子α(TNF-α)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的猴肿瘤坏死因子α(TNF-α)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Monkey TNF-α. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Monkey TNF-α present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
危险品化学品经营许可证（带存储）