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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
脂联素(又称 GBP-28、apM1、AdipoQ 和 Acrp30)是一种蛋白质激素和 脂肪因子,参与调节葡萄糖水平和脂肪酸分解。它由 ADIPOQ 基因编码,主 要在脂肪组织中产生,但也在肌肉中,甚至在大脑中。脂联素是一种 244 个 氨基酸长的多肽(蛋白质)。脂联素有四个不同的区域。首先是一个短的信 号序列,针对激素在细胞外的分泌;接下来是一个短的区域,在不同的物种 之间有所不同;第三个是一个 65 个氨基酸的区域,与胶原蛋白相似;最后 一个是一个球状结构域。总的来说,这种蛋白质显示出与补体 1Q 因子(C1Q) 的相似性。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有猴脂联素(ADPN)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的猴脂联素(ADPN)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Monkey ADPN. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Monkey ADPN present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.