BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Ⅲ型前胶原（PCⅢ）是一种由成纤维细胞、平滑肌细胞等合成的蛋白质前体，其核心功能是参与细胞外基质（ECM）的胶原纤维形成。作为Ⅲ型胶原的前体，PCⅢ在细胞内合成后被分泌至细胞外，经内切酶切割后转化为具有三螺旋结构的Ⅲ型胶原，进而交联形成胶原纤维。PCⅢ在临床中是评估肝纤维化程度的关键指标。其血清水平与肝纤维化程度呈正相关，尤其在肝纤维化早期，PCⅢ率先升高，成为诊断肝纤维化的敏感标志物。持续升高的PCⅢ提示肝脏纤维化处于活动期，但肝硬化晚期时其水平可能因合成率降低而下降。此外，PCⅢ也可用于监测其他器官的纤维化进程，如肺纤维化、肾纤维化等。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有大鼠Ⅲ型前胶原(PC Ⅲ)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠Ⅲ型前胶原(PC Ⅲ)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat PC Ⅲ. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat PC Ⅲ present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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